阿魏酸酯酶与纤维素酶协同水解麦麸释放阿魏酸的研究

Synergistic Effect of Feruloyl Esterase and Cellulase on the Release of Ferulic Acid from Wheat Bran

DOI：10.3969/j.issn.1673-1689.2015.04.007

中文关键词: 阿魏酸酯酶 纤维素酶 淀粉麸皮 协同作用 阿魏酸

英文关键词: feruloyl esterase, cellulase, starched wheat bran, synergistic action, ferulic acid

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中文摘要:

研究了重组阿魏酸酯酶和纤维素酶协同作用水解去淀粉麸皮对阿魏酸释放量的影响。以高效液相色谱法检测阿魏酸的提取量,结果显示,底物的超声预处理最佳条件为350 W,20 min,是未经超声处理的底物阿魏酸释放率的1.45倍,单独使用阿魏酸酯酶(reAoFaeA)30 U时阿魏酸的释放率仅为4.1%,单独使用纤维素酶(rAuCel12A)并不释放阿魏酸,当两种酶协同作用时,阿魏酸的释放率明显提高,经单因素试验确定双酶协同作用的最佳条件为:reAoFaeA的最适添加量为30 U,rAuCel12A的最适添加量为70 U,水解时间为10 h,水解温度为40 ℃,水解pH为5.0,料液质量体积比为1 g:30 mL,此时阿魏酸的释放率为23.6%。该结果表明,去淀粉麸皮中纤维素的降解,对提高阿魏酸酯酶水解释放阿魏酸效率具有重要作用。

英文摘要:

The synergistic effect of feruloyl esterase and cellulose on the release of ferulic acid from de-starched wheat bran (DSWB) was studied. The HPLC results showed that DSWB which was ultrasonically pretreated at a power of 350?W for 20 minutes released ferulic acid at a level of 1.45-fold more than that unpretreated. ReAoFaeA (30 U) by itself released only 4.1% of total alkali-extractable ferulic acid from wheat bran, while no ferulic acid released if rAuCel12A was used. The rate of ferulic acid released was significantly increased when both enzymes were used. The conditions using two enzymes simultaneously were optimized by a single factor test. A maximum of 23.6% total ferulic acid released could be reached in combinations of 30 U reAoFaeA and 70 U rAuCel12A with the ratio of solid to liquid of 1∶30 (g∶mL) at pH 5.0 and treated under 40 °C for 10 h.

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