石榴皮中鞣花酸的抗衰老性能及机理研究

Studies on the Anti-Aging Effect of Ellagic Acid from Pomegranate and Its Mechanism of Action

DOI：10.3969/j.issn.1673-1689.2015.04.017

中文关键词: 鞣花酸 抗衰老 光谱法

英文关键词: ellagic acid, anti-aging, spectroscopy

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中文摘要:

利用光谱法研究了石榴皮中鞣花酸的抗衰老性能和机理。首先,通过分光光度法考察了鞣花酸对DPPH·、·OH和·O2-等3种自由基的清除率和弹性蛋白酶抑制率,结果证明了鞣花酸抗衰老能力强。其次,以弹性蛋白酶为研究对象,利用光谱法探讨了鞣花酸对弹性蛋白酶的作用机理。结果表明,鞣花酸与弹性蛋白酶发生作用并形成络合物,当鞣花酸的质量浓度为4.57 mg/mL时,鞣花酸对弹性蛋白酶的抑制率高达88.26%。当鞣花酸浓度低于4.0×10-5 mol/L时,其荧光猝灭机理主要是静态猝灭,鞣花酸浓度较高时动态猝灭所占比例增加。因此,鞣花酸可以作为天然自由基清除剂与弹性蛋白酶抑制剂应用于抗衰老化妆品中。

英文摘要:

The anti-aging effects and mechanisms of ellagic acid from pomegranate have been studied. Firstly, free radical scavenging activity of ellagic acid was determined according to the elimination of DPPH ·OH and ·O2- radicals by spectrophotometry as well as the elastase inhibition activity. Secondly, the mechanism of interaction between ellagic acid and elastase was investigated with fluorescence spectra. An 88.26% inhibition rate could be reached when the ellagic acid-elastase complex was formed if the concentration of ellagic acid was 4.57 mg mL-1. The fluorescence quenching of elastase when interacted with ellagic acid was regarded as a static quenching when the concentration of ellagic acid was lower than 4.0×10-5 mol L-1. The dynamic quenching increased when raised the ellagic acid concentration. Ellagic acid, a natural free radical scavenger and inhibitor, has great potential in the cosmetics industry as an anti-aging ingredient.

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