甘薯TPS基因家族分析及IbTPS7a基因克隆与功能研究

Analysis of Trehalose-6-Phosphate Synthase（TPS） Gene Family and Identification of IbTPS7ain Sweet Potato（Ipomoea batatas（L.） Lam.）

DOI：10.3969/j.issn.1673-1689.2018.10.015

中文关键词: 关键字： 甘薯 海藻糖 抗逆 数字表达谱

英文关键词: Ipomoea batatas,trehalose,stress resistance,digital gene expression profiling

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中文摘要:

为了研究甘薯TPS基因家族及功能，采用生物信息学手段分析鉴定了甘薯转录组数据库中7个TPS基因，并对甘薯TPS基因家族编码蛋白进行了进化树分析，结果发现7个IbTPS基因可以分成两个亚家族， IbTPS7a蛋白与Nicotiana sylvestris和Solanum tuberosum的TPS7蛋白亲缘关系最接近。利用RT-PCR技术成功克隆得到全长为2 568 bp的IbTPS7a基因，将IbTPS7a基因转化大肠杆菌和酿酒酵母进行过表达，实验结果发现在非生物胁迫下重组菌株的生长趋势或生存率有明显提升。数字表达谱的结果表明IbTPS7a基因在甘薯不同组织中的表达量并不相同，其在茎中的表达量最高，在须根中的表达量最低。以上结果表明甘薯中也存在TPS基因家族， IbTPS7a可以有助于提升细胞在非生物胁迫下的抗逆能力。

英文摘要:

In order to study the structure and function ofTPSfamily genes in Ipomoea batatas，7 IbTPSfamily genes were identified and their sequences，structures and functional properties were determined by bioinformatic method. These IbTPSgenes were divided into class I and class II subfamilies according to their structure and phylogeny relationship. In this study，the complete IbTPS7a gene cloned from Ipomoea batatas contains an open reading frame（ORF ） of 2 568 bp. Meanwhile，IbTPS7awas transformed into E. coli BL21（DE3） andSaccharomyces cerevisiaeINVSc1，respectively. The results indicated that over-expression of IbTPS7a conferred recombinant strains better growth or survival rate than control strain. Digital gene expression profiling（DGE） proved that the expression level of IbTPS7ahas a difference in different tissues，the highest transcription level of IbTPS7awas in stem and the lowest level was in fibrous root. The results indicated that IbTPS7a gene family existed in sweet potato and IbTPS7awas helpful to enhance cell resistance to abiotic stress.

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