银杏叶提取液对腐生葡萄球菌的作用机理

Antimicrobial Mechanism of Ginkgo biloba Leaf Extracts （GBLE） AgainstSaprophytic staphylococcus

DOI：10.3969/j.issn.1673-1689.2018.09.011

中文关键词: 银杏叶提取液 腐生葡萄球菌 抑菌机理

英文关键词: Ginkgo biloba leaf extracts （GBLE）,Saprophytic staphylococcus,antimicrobial mechanism

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中文摘要:

为研究银杏叶提取液对水产品特定腐败菌--腐生葡萄球菌的作用机理，作者采用肉汤稀释和平板计数法测定其对腐生葡萄球菌的最小抑菌浓度（Minimal Inhibitory Concentration，MIC），并通过细菌生长曲线、碱性磷酸酶量、电导率与蛋白质质量浓度，综合评价银杏叶提取液对腐生葡萄球菌菌体生长、细胞壁和细胞膜通透性、完整性的影响；由扫描电镜观察不同处理浓度的银杏叶提取液对腐生葡萄球菌菌体的作用效果。结果得出：银杏叶提取液对腐生葡萄球菌的MIC为100 mg/mL；MIC与2MIC质量浓度下银杏叶提取液对腐生葡萄球菌的抑菌率分别为60.58%和81.34%。提取液作用于腐生葡萄球菌后，菌体生长受到抑制，细胞壁完整性被破坏，细胞膜通透性随之提高；由扫描电镜观察表明，银杏叶提取液处理后的腐生葡萄球菌出现细胞变形、胞间粘结等现象，菌体结构受到影响，最终得出银杏叶提取液主要通过对其菌体细胞膜和细胞壁的破坏实现其抑菌效果。

英文摘要:

In order to investigate the antimicrobial mechanism of Ginkgo biloba leaf extracts （GBLE） against specific spoilage bacteria in aquatic products，minimal inhibitory concentration （MIC） of GBLE againstSaprophytic staphylococcuswas measured by method of broth dilution combined with plate counting in this paper. The impact of GBLE on the growth of bacteria，the permeability of cell membrane and cell wall were also investigated by the methods，such as growth curve，content of alkaline phosphatase （AKP），electrical conductivity and content of protein. The structure of the Saprophytic staphylococcus cells was observed by scanning electron microscope （SEM）. The results showed that the MIC was 100 mg/mL. The inhibitory rates of MIC and 2MIC concentration of GBLE were 60.58% and 81.34% respectively. The growth of bacteria was inhibited and the permeability of cell membrane increased significantly. The result of observation with SEM revealed that the structure of Saprophytic staphylococcus cells were destroyed and the bacteria began to be adhesive with each other. The inhibition effect of GBLE against Saprophytic staphylococcus was related with the damage of cell membrane and cell wall.

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