高产叶酸植物乳杆菌的筛选及应用研究

Screening of Lactobacillus plantarum with High Level Folate and Their Application Researches

DOI：10.3969/j.issn.1673-1689.2018.05.006

中文关键词: 植物乳杆菌 叶酸 高效液相色谱法 酸奶 发酵大豆

英文关键词: Lactobacillus plantarum,folate,high performance liquid chromatography,yoghurt,fermented soybean

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中文摘要:

人类自身不能合成叶酸，主要从食物中摄取。植物乳杆菌（Lactobacillus plantarum，Lb. plantarum）能够产生叶酸，且是公认的肠道益生菌。本研究利用高效液相色谱检测方法，从34株分离自云南传统发酵食品的植物乳杆菌中筛选出高产叶酸植物乳杆菌，结果显示，34株植物乳杆菌均具有合成叶酸的能力，其中Lb. plantarum SP8-7，JH-M1-6-30和YML4-3三株菌的叶酸产量最高，分别为1.492，1.360 μg/mL和1.312 μg/mL。将其作为发酵菌株制作酸奶和发酵大豆，Lb .plantarum JH-M1-6-30与Lb. plantarum YML4-3可以联合用于富含叶酸酸奶的制作，而Lb. plantarum SP8-7可以用于制作富含叶酸的发酵大豆。

英文摘要:

The human being can not synthesize folate by themselves，mainly from food. Lactobacillus plantarum（Lb. plantarum） can produce folate，and recognized as an intestinal probiotics. Studying higher-yield folate Lb. Plantarum，which isolated from Yunnan traditional fermented food，totaled 34 strains，combining high performance liquid chromatography（HPLC）. Through comparing，the 34 Lb. Plantarum also have the ability to synthesize folate，and the three highest yields are Lb. plantarum SP8-7，JH-M1-6-30 and YML4-3，1.492，1.360 μg/mL and 1.312 μg/mL respectively. In the aspect of applied research，Lb. plantarum JH-M1-6-30 and Lb. plantarum YML4-3 can be used in producing yoghurt which containing higher-yield folate，and Lb. plantarum SP8-7 can be used in producing acid douchi which containing higher-yield folate.

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