灵芝孢子粉多糖的分离纯化、结构表征及免疫活性初探

Purification，Structural Characterigation and Immunological Activity of Polysaccharides from the Spores of Ganoderma lucidum

DOI：10.3969/j.issn.1673-1689.2018.05.008

中文关键词: 灵芝孢子粉多糖 分离纯化 结构表征 免疫活性

英文关键词: Ganoderma lucidum spore polysaccharides,purification,structural characterigation,immunological activity

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中文摘要:

采用水浴回流法提取灵芝孢子粉粗多糖，再依次使用DEAE Sepharose Fast Flow离子交换柱和Sepharose CL-6B凝胶色谱柱分离纯化得到2种多糖组分GLP1a和GLP1b，HPSEC法测得2种组分均呈单一峰，重均分子量分别为1.12×106和1.21×105。HPLC、红外光谱和核磁共振分析的结果表明，GLP1a和GLP1b主要由葡萄糖组成，主链都是以β-（1，6） 糖苷键连接，GLP1b在部分1→6连接的葡萄糖的3位和4 位有分支。MTT实验表明，2个多糖级分均具有一定的免疫活性，而且都存在明显的量效关系，GLP1b的免疫活性明显高于GLP1a。

英文摘要:

The crude polysaccharides was obtained from the hot-water extract of the spores of Ganoderma lucidum，and two main fractions GLP1a and GLP1b were isolated by DEAE Sepharose Fast Flow chromatography and Sepharose CL-6B gel chromatography. The HPSEC analysis indicated that the two fractions were all homogeneous polysaccharides and their velative molecular mass weight were respectively 1.12×106 and 1.21×105. The analysis of composition，infrared spectrum and NMR spectroscopy indicated that GLP1a and GLP1b all had a backbone of β-D-（1，6） -glucan and GLP1b were partly substituted at C-3 and C-4 in the main chain. Preliminary tests in vitro showed that the two polysaccharide fractions all has stimulating effects on murine splenocyte proliferation and the effect of GLP1b was better than GLP1a. Moreover，their activities were all in a dose-dependent manner. GLP1a.

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