HPLC-ELSD测定灵芝及其孢子粉中胆碱质量分数

Determination of Choline in Ganoderma lucidum and Its Spore Powder by HPLC-ELSD

DOI：10.3969/j.issn.1673-1689.2018.01.006

中文关键词: HPLC_ELSD 灵芝 孢子粉 胆碱

英文关键词: HPLC-ELSD,Ganoderma lucidum,spore powder,choline

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中文摘要:

建立了一种运用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定灵芝及其孢子粉中胆碱含量的方法。实验采用Agilent公司ZORBAX 300-SCX阳离子交换色谱柱（4.6 mm×250 mm，5 μm），柱温30 ℃，流动相为乙腈-乙酸铵溶液，等度洗脱，流量为1.0 mL/min，进样量为10 μL；蒸发光散射检测器检测，漂移管温度为60 ℃，喷雾器为模式加热，雾化气为净化空气，气体压力为172.4 kPa。研究结果表明，该方法精密度、重复性、稳定性和回收率均良好，在6.25～200 μg/mL范围内胆碱具有良好的线性关系，检出限为6 μg/mL，定量限为12 μg/mL，是一种快速高效检测灵芝及其孢子粉中胆碱的方法。

英文摘要:

To establish an HPLC-ELSD method for determining the content of choline in Ganoderma lucidum spore powder. The experiment using Agilent company ZORBAX 300-SCX cation exchange chromatography column（4.6 mm×250 mm，5 μm）as the stationary phase，the column temperature was set at 30 ℃，the mobile phase was 0.3% acetonitrile - ammonium acetate solution（60∶40），with isocratic elution，the flow rate was 1.0 mL/min，the injection volume was 10 μL，the detector was evaporative light -scattering detector（ELSD），the drift tube temperature of the ELSD was set at 60 ℃，the spray mode was heated，and the purifying air pressure was 172.4 kPa. Research results show that the method precision，repeatability，stability and recovery rate are good.The choline showed a good linear relationship in the range of 6.25～200 μg/mL. The limit of detection was 6 μg/mL，and the limit of quantitation was 12 μg/mL.The results show tant it is a fast and efficient analysis method to determinate the content of choline in Ganoderma lucidum spore powder .

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