不同来源副溶血性弧菌耐药情况比较

Comparison of Antimicrobial Resistance of Vibrio Parahaemolyticus Isolated from Different Sources

DOI：10.3969/j.issn.1673-1689.2019.12.002

中文关键词: 副溶血性弧菌 耐药性 致病性 菌株来源

英文关键词: V. parahaemolyticus,antimicrobial resistance,pathogenicity,source of strain

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中文摘要:

为了比较不同来源以及不同致病性副溶血性弧菌耐药性差异，通过改良K-B纸片法对44株副溶血性弧菌进行抗生素耐药性检测，应用PCR技术对其耐药基因（Antibiotic resistance genes，ARGs）进行检测。结果表明：从海水虾和淡水虾分离的副溶血性弧菌菌株的耐药表型无明显差异，其耐药率分别为100%和95.5%；但是致病性菌株多重耐药性高达73.7%，显著高于非致病性菌株（其为16%）；不同来源以及不同致病性菌株所含耐药基因种类和数量并无显著差异。本研究为副溶血性弧菌耐药多样性普查提供基础数据，并对进一步研究耐药菌风险评估提供相关的帮助。

英文摘要:

In order to compare the differences in antimicrobial resistance between different sources and different pathogenicVibrio parahaemolyticus，the antibiotic resistance of 44 V. parahaemolyticus strains was detected by modified K-B disk method，and antimicrobial resistance genes（ARGs） were detected by PCR.The results showed that there were no significant differences in antimicrobial resistance phenotype between seawater and freshwater shrimp isolates，and the antimicrobial resistant rates were 100% and 95.5% respectively；However，the multidrug resistance of pathogenic strains reached 73.7%，significantly higher than that of non-pathogenic strains（16%）.The type and number of ARGs between different sources and different pathogenic strains also has no significant differences. This study provides the basic data for the drug resistance diversity of V. parahaemolyticus，and provides relevant help for further study of antimicrobial resistant bacteria risk assessment.

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