铁钉菜水提醇沉上清液部分的抗氧化、抗酪氨酸酶活性及其冲剂的降糖作用

Antioxidant and Anti-Tyrosinase Activities of Supernatants from Water Extracting-Alcohol Precipitation ofIshige okamuraiand Hypoglycemic Effect of Its Granules

DOI：10.3969/j.issn.1673-1689.2019.11.020

中文关键词: 铁钉菜 水提醇沉上清液部分 抗氧化 抗酪氨酸酶 冲剂 降血糖

英文关键词: Ishige okamuraiYendo,supernatants from water extracting-alcohol precipitation,antioxidant activity,anti-tyrosinase,granules,hypoglycemic

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中文摘要:

为探讨海藻铁钉菜提取物的抗氧化、抗酪氨酸酶活性与降糖作用，作者采用DPPH、羟基自由基清除体系测定铁钉菜水提醇沉上清液部分（SWEAPI）的抗氧化活性，采用多巴色素法测定其抗酪氨酸酶活性，并以可溶性淀粉、甜蜜素、β-环状糊精为辅料，采用正交实验设计的优化条件制备铁钉菜固体冲剂；通过体外α-葡萄糖苷酶活性抑制实验与体内四氧嘧啶致糖尿病小鼠模型，研究该冲剂的降血糖作用。结果表明：SWEAPI具有较强的自由基清除能力与抗酪氨酸酶活性，其DPPH自由基清除的半效应质量浓度EC₅₀为1.19 mg/mL，羟基自由基清除的EC₅₀为5.08 mg/mL，酪氨酸酶活性抑制的半效应质量浓度IC₅₀为1.27 mg/mL；这可能与其较高的多酚含量（8.30%）有关。采用SWEAPI：可溶性淀粉：甜蜜素：β-环状糊精为30∶60∶1∶7.5的配比进行铁钉菜冲剂制备可获得较好的产品质量，冲剂的水分质量分数≤10%、总糖质量分数约为18.74 %、蛋白质质量分数16.60%、多酚质量分数2.53%。该冲剂具有较强的α-葡萄糖苷酶抑制活性，半效应质量浓度IC50为0.37 mg/mL。口服冲剂14 d后，与模型组比较，350 mg/kg的使用剂量时可极显着降低糖尿病小鼠血糖，糖耐量实验进一步证实其可以在短时间内降低餐后血糖，总体上，其降糖效果与阳性药物二甲双胍处理组（500 mg/kg）相近。可见，SWEAPI具有较强的抗氧化活性、抗酪氨酸酶活性与降糖作用，具有开发成海藻源护肤品、降糖功能产品的潜在应用价值。

英文摘要:

The study aimed to investigate the antioxidant，anti-tyrosinase，and hypoglycemic activities of the extracts fromIshige okamurai.The antioxidant activities of the supernatants from water extracting-alcohol precipitation ofIshige okamurai（hereafter，referred to as simply SWEAPI） were determined by the DPPH and hydroxyl radical scavenging system，its anti-tyrosinase activity was determined by the dopachrome method. Using SWEAPI as basic material and soluble starch，sodium cyclamate，and β-cyclodextrine as auxiliary materials，the granules were prepared by the optimization conditions from orthogonal experiment. The hypoglycemic effects of granules were studied byin vitroα-glycosidase enzyme activity inhibition experiment andin vivoalloxan-induced diabetic mice. The results show that EC50 of DPPH scavenging is 1.19 mg/mL，EC50 of hydroxyl radical scavenging is 5.08 mg/mL，and IC50 of tyrosinase inhibition is 1.27 mg/mL. These phenomena are related to its higher polyphenol content（8.30%）. When the granules are made by the optimization conditions with the 30∶60∶1∶7.5 ratio of SWEAPI：soluble starch：sodium cyclamate：β-cyclodextrine，it has the best product quality with content of water（≤10%），total sugar（18.74 %），protein（16.60%），and polyphenol（2.53%）. The granules have strong inhibitory activity against α-glycosidase enzyme with IC50 of 0.37 mg/mL. Compared with the model control group，the granules could significantly reduce the blood glucose level of diabetes mice after 14 days of treatment with oral dose of 350 mg/kg. The granules also could reduce postprandial blood glucose level in a short time by the results of glucose tolerance experiment，which are similar to positive drug metformin at dose of 500 mg/kg. In conclusion，SWEAPI has strong antioxidant，anti-tyrosinase，and hypoglycemic activities with potential applications in skin care products and hypoglycemic functional products.

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