蛹虫草固态发酵产虫草素的优化

Optimization of Cordycepin Production by Solid-State Fermentation ofCordyceps militaris

DOI：10.3969/j.issn.1673-1689.2019.10.005

中文关键词: 蛹虫草 固态发酵 虫草素

英文关键词: Key ords：Cordyceps militaris,solid-state fermentation,cordycepin

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中文摘要:

为提高虫草素的产量，本实验对蛹虫草固态发酵产虫草素进行优化。通过一系列单因素实验，确定大米为发酵基质，葡萄糖和黄豆粉分别为最适碳源和氮源，得到最佳培养基组成和最佳培养条件：大米30 g（粒径0.90~1.25 mm），料液比（m/v）1∶1.5，葡萄糖3%（按基质算，下同），黄豆粉2%，麦麸1%，接种量30%，种龄2 d，发酵时间12 d。优化后虫草素产量达到4.69%，约为优化之初（0.74%）6.34倍。

英文摘要:

To improve the production of cordycepin，the experiments were conducted to optimize the production of cordycepin by solid-state fermentation ofCordyceps militaris. Through a series of single-factor experiments，rice was the fermentation substrate，glucose and soybean flour were the optimum carbon source and nitrogen source respectively，the optimum medium compositions and culture conditions were：rice 30 g（particle size 0.90~1.25 mm），solid-liquid ratio（m/v）1∶1.5，glucose 3%（based on substrate，the same below），soybean flour 2%，wheat bran 1%，inoculation amount was 6 mL，seed age was 2 d，fermentation time was 12 d. The optimization results are that cordycepin production reached 4.69%，which is about 6.34 times compared with the initial level（0.74%）.

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