一株锌抗性菌株强化印度芥菜修复锌污染土壤的可行性研究

Feasibility of Enhanced Phytoextraction of Zn for Contaminated Soil with a Zinc-Resistant Strain

DOI：10.3969/j.issn.1673-1689.2019.10.008

中文关键词: 锌促溶作用 根际促生菌 印度芥菜 促生效果 植物修复

英文关键词: zinc solubilization,plant growth promoting rhizobacteria,Indian mustard,growth promoting effect,phytoremediation

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中文摘要:

对一株从土壤中筛选得到的锌抗性菌株微紫青霉菌（Penicillium janthinellum）BC109-2促进印度芥菜对土壤中Zn的修复效果进行评价。分析了菌株BC109-2对难溶态锌的促溶作用及产铁载体和吲哚乙酸（IAA）能力，并对其1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性和溶磷能力进行测定，再通过印度芥菜种子根伸长量实验和盆栽试验评价了锌抗性菌株BC109-2对植株生长和锌提取效率的影响。结果表明：菌株BC109-2具有明显的溶解碳酸锌的能力，与接种灭活菌的对照相比，菌株BC109-2使培养液中水溶性Zn含量增加了213%，培养液的pH由初始的7.0降低到5.7。另外该菌株能产IAA和铁载体，并且具有ACC脱氨酶活性和溶磷能力，充分表明菌株BC109-2是一株植物根际促生菌（PGPR）。根际促生菌BC109-2能促进植物根系伸长，伸长率在4.14%~44.83%之间，使印度芥菜生物量得到不同程度的增加；接种根际促生菌BC109-2的印度芥菜对土壤中锌的富集量较对照提高1.01~1.46倍。研究结果表明，微紫青霉菌BC109-2兼具多种促生特性且对印度芥菜有较好的促生效果，可用于提高植物修复锌污染土壤的效率。

英文摘要:

The effects of zinc-resistant strainPenicillium janthinellumBC109-2 on the promoting of zinc uptake in soil by Indian mustard were evaluated. The promoting solubility of insoluble zinc was analyzed. The abilities of producing siderophore and indole acetic acid（IAA），activities of amino cyclopropane-1-carboxylic acid（ACC） deaminase and the ability of phosphate dissolution were determined. Through the root elongation experiment of Indian mustard seed and pot experiment，the effects of zinc resistant strain BC109-2 on plant growth and the efficiency of zinc uptake were evaluated. The results showed that the strain BC109-2 had the obvious ability of dissolving zinc carbonate. Compared with the control treatment，the strain BC109-2 made the water-soluble Zn content in nutrient solution increase by 213% and the pH values in nutrient solution decrease from 7.0 to 5.7. In addition，this strain could produce IAA and siderophore and had ACC deaminase activity and phosphorus dissolving ability，which indicated that strain BC109-2 was a plant growth promoting rhizobacteria（PGPR）. The strain promoted the root elongation of Indian mustard. The root elongation ratio was between 4.14%~44.83%. The strain induced the increase of India mustard biomass in different degrees. The zinc accumulation in India mustard inoculated with the strain BC109-2 increased by 1.01~1.46 times compared with the control. This study demonstrated that Penicillium janthinellum BC109-2 possessed a variety of growth promoting properties and had good growth-promoting effect on India mustard. The PGPR BC109-2 might be used for enhancing remediation efficiency for the soil polluted by zinc.

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