RNAi 靶向沉默opaque2基因对高粱醇溶蛋白含量的影响

Effects of RNAi Targetingopaque2 Gene Expression on the Kafirin Content in Sorghum

DOI：10.3969/j.issn.1673-1689.2019.10.011

中文关键词: 高粱 RNA i opaque2基因 醇溶蛋白 含量

英文关键词: sorghum,RNA interference,opaque2,kafirin,content

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中文摘要:

为研究opaque2基因沉默对高粱醇溶蛋白含量的影响，采用RNAi技术构建opaque2基因抑制表达载体，利用农杆菌介导转化高粱幼胚，测定转基因植株籽粒中醇溶蛋白含量，SDS-PAGE电泳检测醇溶蛋白带型，并使用透射电镜观察胚乳细胞中蛋白质体变化。结果表明，与野生型相比，5株转基因植株中4株籽粒的醇溶蛋白含量显著降低（Popaque2基因沉默引起胚乳细胞蛋白质体数量变少，体积变小，进而影响胚乳细胞内部结构和籽粒醇溶蛋白含量。

英文摘要:

In order to explore the effect of silencingopaque2 gene on the kafirin content in sorghum，the suppressed expression vector ofopaque2 gene is constructed by RNAi technology，and then transformed to young embryos byAgrobacterium tumefaciens-mediated transformation system. The kafirin content in these transgenic seeds is determined and indentified by SDS-PAGE，and also the changes of protein bodies in these endosperm cells are observed by transmission electron microscope. The results show that the kafirin content of four fifths of transgenic plants is significantly lower than that of the wild type（Popaque2 gene silencing induced changes in number and volume of protein bodies in endosperm cells，which additionally affects the internal structure of endosperm cells and the kafirin content in sorghum.

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