植物乳杆菌FB-T9抑制变异链球菌及其生物膜形成的研究

Inhibition of Streptococcus mutans and Its Biofilm Formation byLactobacillus plantarumFB-T9

DOI：10.3969/j.issn.1673-1689.2019.09.003

中文关键词: 乳酸菌 变异链球菌 龋病 抑菌性 生物膜

英文关键词: Lactic acid bacteria,Streptococcus mutans,dental caries,antibacterial activity,biofilm

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中文摘要:

为筛选得到对变异链球菌及其生物膜形成具有抑制作用的益生菌，从健康及龋病病人口腔、健康人粪便以及新鲜菜叶等样品中分离出乳酸菌，从中挑选出12株对变异链球菌具有良好抑菌作用的菌株进行抑制变异链球菌生物膜形成实验，并对这些菌株的共聚和自聚能力、自身生物膜形成能力、表面疏水性、酸碱电荷等性质进行测定。通过PCA综合分析得到一株性能优良的植物乳杆菌FB-T9，并探究了FB-T9在不同时期介导对变异链球菌生物膜形成的影响。为考察FB-T9在口腔环境中的生存适应性，对该菌粘附唾液包裹的羟磷灰石的能力和溶菌酶的耐受能力进行了体外分析。结果表明植物乳杆菌FB-T9具有良好的抑制变异链球菌及其生物膜形成的能力，同时表现出较理想的自聚和共聚能力、表面疏水性质。菌体表面酸电荷较低、碱电荷较高，自身生物膜形成能力低。对唾液包裹的羟磷灰石的粘附率为32.93%，溶菌酶最高耐受质量浓度为1.2 mg/mL，说明植物乳杆菌FB-T9具有作为预防龋病的口腔益生菌的潜力。

英文摘要:

In order to screen probiotics that had a inhibitory effect on Streptococcus mutans and its biofilm formation，lactic acid bacteria were isolated from oral samples and feces of healthy people and fresh vegetable leaves，among which 12 strains that could significantly inhibit the growth ofS. mutanswere selected to detect their abilities of inhibiting the biofilm formation ofS. mutans.The abilities of auto-aggregation，co-aggregation，self-biofilm formation，surface hydrophobicity and surface acid-base charge of these strains were tested，and finallyLactobacillus plantarumFB-T9 was selected by PCA analysis. The inhibition of biofilm formation ofS. mutansby FB-T9 at different time was further studied. To investigate the survival adaptation of FB-T9 in oral environment，the adhesive capacity to saliva-coated hydroxyapatite and lysozyme tolerance of FB-T9 were measured.L. plantarumcould strongly inhibit the growth ofS. mutansand its biofilm formation and showed ideal characters. The adhesive capacity of this strain was 32.93% and the maximum tolerated concentration of lysozyme was 1.2 mg/mL. The results showed thatL. plantarumFB-T9 could be a potential probiotic to prevent dental caries.

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