糖醇的HPLC-ELSD法测定及其热稳定性研究

Simultaneous Determination of Sugar Alcohols by HPLC-ELSD and the Sugar Alcohol Thermal Stability

DOI：10.3969/j.issn.1673-1689.2019.09.020

中文关键词: 关键字： 糖醇 HPLC-ELSD 定量检测 热稳定性

英文关键词: sugar alcohols,HPLC-ELSD,quantitative detection,thermal stability

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中文摘要:

采用高效液相-蒸发光散射检测器（HPLC-ELSD）法对4种糖醇（赤藓糖醇、木糖醇、山梨醇、麦芽糖醇）进行了同时检测分析并研究了其热稳定性。较佳的检测条件为Amide 80亲水柱，流量0.2 mL/min，柱温33 ℃，ELSD漂移管温度50 ℃，载气流量2.5 L /min。结果表明，4种糖醇的进样量在0.025～1.000 mg/mL范围内，线性关系良好.赤藓糖醇、木糖醇、山梨醇、麦芽糖醇的检测限分别为14.1、33.0、19.5、15.3 μg/mL，精密度（相对标准偏差RSD）分别为1.55 %、1.23 %、1.30 %、1.49 %。在不同加热温度和加热时间下，4种糖醇的热稳定性具有如下规律：木糖醇＞赤藓糖醇＞山梨醇＞麦芽糖醇。

英文摘要:

Four kinds of sugar alcohols（erythritol，xylitol，sorbitol and mailitol） were analyzed by high performance liquid-evaporative light scattering detector（HPLC-ELSD） and the thermal stability was also studied. The optimum conditions were Amide 80 hydrophilic column，flow rate 0.2 mL/min，column temperature 33 ℃，ELSD drift tube temperature 50 ℃，carrier gas flow rate 2.5 L/min. The results showed that the linearity of the four kinds of sugar alcohols was well in the range of 0.025~1.000 mg/mL. The detection limits of erythritol，xylitol，sorbitol and maltitol were 14.1、33.0、19.5、15.3 μg/mL，and the precision（relative standard deviation） was 1.55 %，1.23 %，1.30 % and 1.49%，respectively. The thermal stability of four sugar alcohols under different heating temperature and heating time had the following rules：xylitol＞ erythritol＞ sorbitol＞ maltitol.

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