豆粕蛋白在小鼠胃肠道内的吸收与分布研究

Absorption and Distribution of Soybean Meal Protein in Mice Gastrointestinal Tract

DOI：10.3969/j.issn.1673-1689.2019.08.008

中文关键词: 豆粕 小鼠 酶联免疫 免疫金银染色 分布 吸收

英文关键词: soybean meal,mice,enzyme linked immunosorbent assay,immunogold-silver staning,distribution,absorption

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中文摘要:

探讨一次性灌胃豆粕，不同时间段豆粕蛋白在小鼠胃肠道内的吸收与分布情况。以豆粕中的β-伴大豆球蛋白作为标记，采用双抗体夹心酶联免疫吸附试验技术检测豆粕在小鼠胃肠道内不同部位的吸收量，并采用免疫金银染色技术，对其主要成分豆粕蛋白在胃肠道的分布位置进行示踪，明确其吸收途径。结果表明，当小鼠摄食豆粕0.5~1 h后，豆粕蛋白主要分布于小肠内，部分于胃内，较少量残余豆粕蛋白分布于大肠内，且于摄食2~4 h，豆粕蛋白在小鼠胃肠道内的吸收趋于稳定，这与普通蛋白的吸收位置和途径是一致的。采用稳定的食物蛋白质作为标记进行示踪，为研究食物在机体胃肠道内的吸收、分布情况提供了新的思路。

英文摘要:

The absorption and distribution of soybean meal protein in mice gastrointestinal tract in different time periods after single dose of soybean meal were investigated. In this study，the protein in soybean meal was used as a marker，to study the absorption of soybean meal in the gastrointestinal tract of mice. Amount of soybean meal at each part of the gastrointestinal tract was quantified by double antibody sandwich enzyme linked immunosorbent assay. The distribution of soybean protein in the gastrointestinal tract was located by immunogold silver staining. The results showed that the soybean meal protein mainly distributed in the small intestine after 0.5—1 h uptake，some in the stomach，and a few soybean meal protein was found in the large intestine，and after 2—4 h，the soybean meal protein distribution tends to be stable，which was consistent with the absorption positions and pathway of protein. Furthermore，using a stable food protein as a marker could be a new idea for studying the absorption and distribution of the food in the gastrointestinal tract.

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