食品及其生产环境和包装材料中分离霉菌的形态与分子鉴定
Morphological and Molecular Identification of Molds Isolated from Food and Their Production Environments and Packaging Materials
DOI:10.3969/j.issn.1673-1689.2019.06.007
中文关键词: 霉菌 鉴定 形态学 分子鉴定 食品
英文关键词: molds,identification,morphology,molecular characterization,food
基金项目:
作者
单位
王双霞
上海海洋大学 食品学院,上海 201306
唐晓阳
康师傅控股中央研究所食品安全研究中心检验科学部,上海 201103
范艳红
丁艳杰
谭洋岚
中国科学院上海生命科学研究院 营养科学研究所,上海 200031
胡东强
田野
赵勇
武爱波
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中文摘要:
为了明确食品及食品包装材料、食品的生产坏境中污染霉菌菌株种类,本文对收集、单孢分离的55株霉菌菌株同时采用形态学和分子生物学方法鉴定,并相互验证。实验结果表明:55株污染霉菌主要包括有曲霉属(Aspergillussp.)、镰刀菌属(Fusarium sp.)、青霉属(Penicilliumsp.)、枝孢菌属(Cladosporiumsp.)、链格孢属(Alternariasp.)等,这为食品中霉菌污染的安全控制和菌株溯源提供了有价值的参考信息。
英文摘要:
In order to unveil the population structure of the mold strains which may cause contamination in food and their production environments and packing materials,the 55 isolates were first collected and mono-spore purified from the collected samples,and then the morphological and molecular characterization means were both conducted to identify the mold strains to the species level. The experimental results have indicated that the 55 isolates of mold species identified mainly includeAspergillus,Fusarium,Penicillium,CladosporiumandAlternaria species,etc. These achieved data would be valuable references for safety control and monitoring of contamination of mold species.
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