荞麦蜂花粉多糖A的分离及其降血糖活性

Separation of Polysaccharide A from Buckwheat Bee Pollen and Its Hypoglycemic Activity

DOI：10.3969/j.issn.1673-1689.2019.06.011

中文关键词: 荞麦蜂花粉 多糖 分离 降血糖 化学结构

英文关键词: buckwheat bee pollen,polysaccharide,separation,hypoglycemia,chemical structure

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中文摘要:

对荞麦蜂花粉粗多糖进行分离鉴定并检测纯化多糖的降血糖活性。利用DEAE-52离子交换柱、HW-55F、Sephacryl S-400凝胶柱对荞麦蜂花粉粗多糖进行分离，得到一个单一组分多糖A。利用STZ糖尿病小鼠模型检测多糖A的降血糖活性并通过核磁方法对其进行1H-NMR、13C-NMR和135DEPT-NMR分析。结果表明：与高血糖模型组相比，多糖A高、低剂量组的STZ糖尿病小鼠空腹血糖值都显著降低（P＜0.01，P＜0.01），降低率分别为18.46 %、15.11 %，并提高糖尿病小鼠糖耐量。初步推测多糖A主链是（1→6）-β-葡萄糖组成的，在侧链上连有氨基酸。

英文摘要:

The polysaccharides from buckwheat bee pollen were isolated and identified，and the hypoglycemic activity of purified polysaccharides was determined. By using DEAE-52 ion-exchange column，HW-55F gel column and Sephacryl S-400 gel column，polysaccharide A was separated and purified from the crude polysaccharides. The hypoglycemic activity of polysaccharides A was evaluated through the mouse model of diabetes and polysaccharides A was identified by using nuclear magnetic resonance technique. Compared with control group（without treatment of polysaccharide A），the blood glucose in diabetic mice was notably decreased with treatment of polysaccharide A at both high and low doses，with decreased rates of 18.46% and15.11%，respectively. Moreover，polysaccharide A improved glucose tolerance in diabetic mice and its main chain was deduced to be composed of （1 → 6） -β- glucose and the side chains are linked with amino acids.

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