大种量流加式高体积分数乙醇发酵工艺的研究

Study on The Process of Fed-Batch Very High Gravity Ethanol

DOI：10.3969/j.issn.1673-1689.2019.06.015

中文关键词: 高体积分数乙醇发酵 残糖 糖醇转化率 酿酒酵母

英文关键词: very high gravity,residual sugar,sugar-ethanol conversion rate,S. cerevisiae

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中文摘要:

间歇式高体积分数乙醇发酵工艺存在残总糖高、糖醇转化率低的问题。通过对残总糖组成分析发现，糊精和还原糖质量浓度随拌料质量浓度的变大而逐步上升。结合文献分析可知，糊精和还原糖量较高的原因主要与糖化酶活力和酵母对糖的利用能力有关。为了解决这一问题，采用大种量流加式高体积分数乙醇发酵工艺，并对接种量、初始流加时间、流加时长和流加方式进行了优化。结果表明，当接种量（体积分数）为30%，培养8 h后开始流加，流加时长为20 h，且采用匀速的方式流加时，乙醇发酵结果最优。与间歇式高体积分数乙醇发酵工艺比较，残糖降低了6.5 g/L，糖醇转化率提高了3.2%。

英文摘要:

Very high gravity（VHG） batch ethanol fermentation has the problems of high residual sugar concentration and low sugar-ethanol conversion rate. It was found that the concentrations of dextrin and reducing sugar increased gradually with the increase of the concentration of the mixture. The reasons for the high concentration of dextrin and reducing sugar were mainly related to the activity of saccharification enzyme and the sugar utilization ability of yeast. To resolve these problems，process of fed-batch very high gravity ethanol fermentation with massive inocula was proposed in the study，and the inoculum concentration，initial feeding time，feeding duration and feeding mode were optimized. Results showed that the optimum process is 30% inoculation concentration，the initial feeding time after inoculation 8 h，the feeding duration 20 h and constant feeding speed. Compared with the batch fermentation，the residual sugar decreased by 6.5 g/L，meanwhile，the sugar-ethanol conversion rate of increased by 3.2%.

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