使用GC/MS和GC-O鉴定皖南烟叶主要呈香组分

Identification of Main Aroma Compounds in Tobacco Leaves from Southern Anhui Using GC/MS and GC-O

DOI：10.3969/j.issn.1673-1689.2019.06.019

中文关键词: GC-MS/O 皖南烟叶 香气成分 主要呈香物质

英文关键词: GC-MS/O,southern Anhui tobacco leaves,aroma compounds,main odorants

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中文摘要:

皖南烟叶具有焦甜香独特风格。使用顶空固相微萃取（HS-SPME）和同时蒸馏萃取（SDE）提取烟叶香气物质，结合GC/MS和嗅觉测量法（GC-O）对皖南烟叶中的主要呈香组分进行了鉴定。SDE法得到了26个呈香物质，HS-SPME法得到了22个呈香物质。进一步测定了呈香物质的阈值和香气活度值（OAV），最终得到了16个皖南烟叶主要呈香物质（14个OAV值大于1的物质以及茄酮和巨豆三烯酮）。4-乙烯基愈创木酚的香气强度最高，苯乙醛、芳樟醇等物质的香气强度也比较高。2-戊基呋喃、甲基环戊烯醇酮和2-糠醛可能是皖南烟叶焦甜香的主要贡献者。

英文摘要:

Caramel-like aroma，the unique style of southern Anhui tobacco. The aroma components of tobacco leaves from southern Anhui were extracted with head space-solid phase extraction（HS-SPME） and simultaneous distillation and extraction（SDE） and analyzed by GC/MS and GC-O. A total of 26 and 22 aroma compounds were obtained from SDE and HS-SPME，respectively. The thresholds and odor activity values were subsequently measured and the aroma intensity of aroma compounds were evaluated. Finally，16 significant aroma constituents were obtained including 14odorants with OAV higher than 1，solanone and megastigmatrienone. 4-Vinyl-2-methoxy-phenol showed the highest odor intensity，followed by phenylacetaldehyde and linalool. Furthermore，2-pentylfuran，methyl cyclopentenolone and 2-furfural were suspected the main contributor of caramel-like aroma of southern Anhui tobacco.

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