乳酸钠对单增李斯特菌生物被膜形成的抑制作用

Inhibition Effect of Sodium Lactate on Biofilm Formation byListeria monocytogenes

DOI：10.3969/j.issn.1673-1689.2019.03.003

中文关键词: 单增李斯特菌 生物被膜 乳酸钠

英文关键词: Listeria monocytgenes,biofilm,sodium lactate

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中文摘要:

为初步研究乳酸钠对单增李斯特菌生物被膜形成的抑制作用，作者首先采用结晶紫染色法检测不同质量浓度乳酸钠（0、2.5、5、10、20 g/dL）对单增李斯特菌生物被膜形成的抑制效果，其次探究乳酸钠对其生物被膜结构、胞外多糖和胞外蛋白、膜内细菌细胞活性及细胞膜完整性的影响，并采用荧光实时定量PCR（quantitative real-time PCR，qRT-PCR）检测其生物被膜相关基因motB、mogR、degU、flgE、dnaK、prfA及sigB的表达水平。结果表明，随着乳酸钠浓度的增高（2.5%~20%），其对单增李斯特菌生物被膜形成的抑制效果显著增强（p

英文摘要:

The objective of this study was to investigate the inhibition effet of sodium lactate（NaL） on biofilm formation byListeria monocytogenes. First of all，the crystal violet staining assay was used to evaluate the inhibition effect of NaL at different concentrations（0%，2.5%，5%，10% and 20%） on the biofilm formation ofL. monocytogenes. Furthermore，the effets of NaL on its biofilm structure，polysaccharides，extracelluar proteins，cell activity and membrane integrity ofL. monocytogenesbiofilm cells were also investigated. Finally，the detection of the expression level of the biofilm-associated genes motB，mogR，degU，flgE，dnaK，prfA and sigB was performed by using quantitative real-time PCR（qRT-PCR）. With the increase of the NaL concentration（2.5~20%），biofilm formation ofL. monocytogeneswas inhibited significantly（pL. monocytogenesbiofilm cells to suppress the development of new biofilms. qRT-PCR results revealed that NaL could downregulate biofilm-associated genes in L. monocytogenes and therefore to inhibit its biofilm formation. In conclusion，this study could provide a scientific basis for the effective inhibition of NaL onL. monocytogenesbiofilm formation.

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