樟芝提取物Fr.3抑制TGF-β1诱导的肝纤维化机制

Study of Mechanisms of Extract Fraction 3 fromAntrodia camphorateAttenuating TGF-β1-Induced Liver Fibrosis

DOI：10.3969/j.issn.1673-1689.2019.03.008

中文关键词: 肝星状细胞 肝纤维化 TGF-β1 樟芝 信号通路

英文关键词: hepatic stellate cell,liver fibrosis,TGF-β1,Antrodia camphorate,signaling pathways

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中文摘要:

探讨樟芝菌丝体正己烷提取物Fr.3组分对TGF-β1诱导的肝星状细胞（CFSC-8B）活化的影响及作用机制。采用TGF-β1诱导CFSC-8B活化，通过MTT、WST-1、小室迁移、qRT-PCR及western blotting法检测Fr.3组分对TGF-β1诱导后的CFSC-8B细胞增值、迁移及细胞外基质的变化，并研究了其对TGF-β1介导的Smad、PI3K、MAPK信号通路中相关蛋白质表达的影响。Fr.3组分的IC50值为54.56 μg/mL；在细胞存活率80%以上范围内，Fr.3组分（

英文摘要:

To investigate the effects of hexane extract fraction 3（Fr.3） fromAntrodia camphorateon hepatic stellate cells（HSCs） activation induced by TGF-β1，as well as mechanisms of its anti-fibrotic action in vitro. We established a cell model of liver fibrosis by using CFSC-8B stimulated by TGF-β1. Cell proliferation，cell migration，extracellular matrix（ECM） production and protein expression in the TGF-β1 signaling pathways were detected by the MTT，WST-1 assay，cell migration assay，qRT-PCR and western blotting analyses. The IC50 value of Fr.3 was 54.56 μg/mL and we found that less than 20 μg/mL of Fr.3 might be safe for CSFC-8B cells. Fr.3（

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