虫草头孢提取物Fr.8组分对HepG-2胰岛素抵抗的改善作用

Ameliorative Effects of Extract Fraction 8 fromCephalosporium sinensison Insulin Resistance of HepG-2 Cells

DOI：10.3969/j.issn.1673-1689.2019.03.016

中文关键词: 胰岛素抵抗 HepG-2细胞 虫草头孢 mRNA表达 蛋白质表达

英文关键词: insulin resistance,HepG-2 cell,Cephalosporium sinensis,mRNA expression,protein expression

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中文摘要:

为探讨虫草头孢正己烷分离组分Fr.8对高胰岛素诱导的HepG-2细胞胰岛素抵抗的改善作用，作者建立了稳定的胰岛素抵抗细胞模型，并检测了Fr.8组分对胰岛素抵抗细胞（HepG-2/IR）的葡萄糖消耗、葡萄糖摄取及相关基因转录和蛋白质表达的影响。结果显示，5 μmol/L胰岛素是诱导细胞成为HepG-2/IR的最佳作用浓度；Fr.8组分在无细胞毒性范围内可显著增加HepG-2/IR对葡萄糖的消耗及摄取；Fr.8（>25 μg/mL） 可明显上调IR、IRS1、IRS2、Glut2、AMPKα mRNA的表达水平，并上调IR、IRS、Glut2、p-AMPKα、p-AKT蛋白质的表达。研究表明，虫草头孢Fr.8可改善HepG-2胰岛素抵抗，其机制可能与胰岛素受体及底物、葡萄糖转运蛋白2、p-AMPKα和p-AKT蛋白的激活有关。

英文摘要:

To investigate the ameliorative effects of extract fraction 8（Fr.8） fromCephalosporium sinensison insulin resistance of HepG-2 cells，in this study，a stable insulin resistance cell model in vitro was established to detect the glucose consumption，glucose uptake，relative mRNA expression and protein expression of Fr.8 on HepG-2/IR cells.The results showed that 5 μmol/L insulin was the optimal concentration to induce HepG-2 cells become HepG-2/IR cells. In the range of non-cytotoxic，Fr.8 could distinctly promote glucose consumption and uptake in HepG-2 / IR cells.Fr.8（>25 μg/mL） significantly upregulated IR，IRS1，IRS2，Glut2，AMPKα mRNA expression and obviously increased IR，IRS，Glut2，p-AMPKα and p-AKT protein expression.Therefore，we provide theoretical basis to demonstrate Fr.8 fromC. sinensisameliorated insulin resistance of HepG-2 cells，which in part，the mechanism may be associated with insulin receptor and substrate，glucose transporter protein 2，the activated protein of p-AMPKα and p-AKT.

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