天麻蜜环提取物的抗炎活性

Anti-Inflammatory Activity of Mycelial Extracts fromArmillaria mellea

DOI：10.3969/j.issn.1673-1689.2019.03.020

中文关键词: 天麻蜜环 抗炎 脂多糖

英文关键词: Armillaria mellea,anti-inflammatory,LPS

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中文摘要:

天麻蜜环是一种具有良好药用功效的传统药食用菌，然而关于天麻蜜环抗炎活性的研究甚少。作者探索了天麻蜜环的抗炎活性，为开发利用天麻蜜环的抗炎功效以及从中寻找新的天然抗炎化合物提供科学依据。采用MTT法检测提取物的细胞毒性，实验结果显示，仅天麻蜜环正己烷、氯仿高剂量提取物（300 μg/mL） 具有轻微细胞毒性（对细胞活力的抑制率分别为10.7%和17%），其余各剂量提取物均无细胞毒性；采用脂多糖（LPS） 刺激小鼠单核巨噬细胞RAW264.7，分别用天麻蜜环正己烷、氯仿、乙酸乙酯、正丁醇提取物（0，30，100，300 μg/mL）干预以检测其抗炎活性，实验结果显示，天麻蜜环正己烷、氯仿、乙酸乙酯提取物均能呈剂量依赖性地抑制LPS诱导的炎症介质一氧化氮（NO）和炎症因子肿瘤坏死因子（TNF-α）、白介素6（IL-6）、白介素1β（IL-1β）的过量分泌，其中乙酸乙酯提取物既具有良好的安全性又有显著的抗炎活性。

英文摘要:

Armillaria melleais a well-known edible and medicinal mushroom，Whereas little information is available in regard to A. mellea's anti-inflammatory activity. In this study we explored the anti-inflammatory activity of n-hexane，chloroform，ethyl acetate and n-butyl alcohol extracts fromArmillaria mellea. Our work will provide a scientific basis for screening new natural anti-inflammatory substances and a new medical application ofArmillaria mellea. MTT colorimetric assay was applied to measure the cytotoxic effects of different extracts. Their anti-inflammatory activities were evaluated via inhibition against production of lipopolysaccharide（LPS）-induced nitric oxide（NO），tumor necrosis factor alpha（TNF-α），interleukin-6（IL-6） and interleukin-1beta（IL-1β） in murine macrophage-like cell line RAW264.7 cells. Cytotoxic assay showed that only n-hexane and chloroform extracts in a high concentration（300 μg/mL） have slight cytotoxic effects（inhibited 10.7% & 17% of cell viability） in RAW264.7 cells. As to the anti-inflammatory activity，except to the n-butyl alcohol extract，the other three extracts all can dose-dependently inhibited LPS-induced NO，TNF-α，IL-6 and IL-1β production. Among them，the ethyl acetate extract own both safty and anti-inflammatory activity.

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