基于2-酮基-D-葡萄糖酸高产的发酵条件优化研究

Optimization of the Fermentation Condition for 2-Keto-D-Gluconic Acid Producing Strain

DOI：10.3969/j.issn.1673-1689.2019.02.004

中文关键词: 沙雷氏菌属 2-酮基-D-葡萄糖酸 发酵优化 溶氧

英文关键词: Serratia sp.,2-keto-D-gluconic acid,fermentation optimization,dissolved oxygen

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中文摘要:

为了实现异维生素C前体2-酮基-D-葡萄糖酸在沙雷氏菌Serratia sp. FMME043中的高效生产，在30 L发酵罐中对补料策略和发酵条件进行了优化，建立了补料分批发酵工艺。最终确定发酵策略为：溶氧控制在40%，在发酵16、22 h及28 h时分别补加64、80 g和96 g硫酸铵，并且当残糖浓度降至15~25 g/L时，分五次等量补加801 g葡萄糖，优化后，发酵44 h，2-KGA的产量和转化率分别达到268.5 g/L和 1.04 g/g，分别比优化前提高了58.4%和9.9%。本研究结果为目前报道的产量和转化率在国内外属领先水平，为2-酮基-D-葡萄糖酸及异维生素C工业化生产打下了坚实的基础。

英文摘要:

In order to achieve the high production of 2-keto-D-gluconic acid（2-KGA） by Serratia sp. FMME043，feeding strategy and fermentation conditions are optimized in 30 L fermentor，and fed batch fermentation is established. The optimal fermentation conditions are as follows：the dissolved oxygen is 40%，the quality of 64，80 g，and 96 g ammonium sulfate is added at 16，22 h，and 28 h，respectively，and the quality of 801 g glucose is supplemented with five times at the residual sugar concentration between 15~25 g/L. Under these conditions，at 44 h，the production and the yield of 2-KGA reached 268.5 g/L and 1.04 g/g，which are 58.4% and 9.9% higher than that not optimized，respectively.

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