基于PCR-DGGE技术对库尔勒香梨“黑头病”病变组织生物群落的初步研究

Preliminary Analysis of Microbial Communities in Korla Fragrant Pear Diseased Tissues by PCR-DGGE Technique

DOI：10.3969/j.issn.1673-1689.2019.02.007

中文关键词: PCR-DGGE 库尔勒香梨 黑头病 18S rDNA

英文关键词: PCR-DGGE,Korla fragrant pear,blackhead disease,18S rDNA

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中文摘要:

为了解库尔勒香梨“黑头病”病变组织中真菌群落结构特征，应用PCR-DGGE技术对库尔勒地区4个不同保鲜库中12个样品真菌群落结构进行了研究。根据DGGE图谱对生物多样性进行了分析。结果表明，所有样品群落结构存在一定的差异，造成差异的原因可能与库尔勒香梨的种植环境有关；不同样品的9号条带在整个病变过程中优势度最高，其代表的菌株，可被认为是导致库尔勒香梨“黑头病”的主要致病菌；对DGGE的优势条带序列分析，同源性最高的微生物属于链格孢属（Alternaria）。该研究初步揭示了库尔勒香梨病变组织中微生物群落的变化规律，为库尔勒香梨“黑头病”的研究提供理论依据。

英文摘要:

To understand the characteristics of fungal community structure in the lesion tissues of Kuerle fragrant pear "Blackhead Disease", the fungal community structure of 12 samples from 4 different fresh-keeping storage in Kuerle area is studied using PCR-DGGE technique. Biodiversity is analyzed by DGGE fingerprint. The results indicat that there is some differencs in the community structure of all samples，which may be related to the planting environment. The 9th stripe in different samples has the highest dominance in the whole the lesion process. The representative strains can be considered as the main pathogenic bacteria in Korla pear "blackhead disease". According to the results of sequence analysis of DGGE dominant bands，it is phylogentically close toAlternaria. It preliminary reveals the variation regular of microbial communities in Korla fragrant pear diseased tissues，which provides a theoretical basis for studies of "Blackhead Disease" in Korla fragrant pear.

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