青钱柳活性成分对IR-HepG2细胞葡萄糖消耗量及α-葡萄糖苷酶活性的影响
Effects ofCyclocarya paliurus(Batal.) Iljinskaja Extracts on Glucose Consumption of Insulin Resistance HepG2 Cell and Activity of
DOI:10.3969/j.issn.1673-1689.2019.02.017
中文关键词: 青钱柳 HepG2细胞 多糖 黄酮 α-葡萄糖苷酶
英文关键词: Cyclocarya paliurus(Batal.) Iljinskaja,HepG2 cell,polysaccharide,flavone,alpha- glucosidase
基金项目:
作者
单位
王胤康
华中科技大学 生命科学与技术学院,湖北 武汉 430074
吕萌
武汉百思凯瑞纳米科技有限公司,湖北 武汉430075
许琦
陈伟鸿
无限极(中国)有限公司,广东 广州 510665
谭开祥
湖北思慧生物科技有限公司,湖北 恩施 445099
向极钎
恩施州农业科学院,湖北 恩施 445099
刘卫
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中文摘要:
研究青钱柳多糖(CPP)和青钱柳黄酮(CPF)对胰岛素抵抗的人源肝癌细胞(IR-HepG2)葡萄糖消耗量及α-葡萄糖苷酶活性影响。用高浓度胰岛素诱导培养HepG2细胞,建立IR-HepG2细胞模型,将IR-HepG2细胞分为模型组、CPP高、中、低剂量组、CPF高、中、低剂量组和二甲双胍组,各组以相应药物孵育24 h,采用葡萄糖试剂盒测定细胞葡萄糖消耗量(ΔGC),MTT法测定单位细胞葡萄糖消耗量(ΔGC/OD);以阿卡波糖为阳性对照,比较不同浓度梯度的CPP与CPF对α-葡萄糖苷酶活性影响。与模型组比较,阴性对照组、二甲双胍组、CPP组与CPF组细胞?驻GC与ΔGC/OD显著性升高;不同浓度的CPP与CPF对α-葡萄糖苷酶均有抑制作用。提示青钱柳活性提取物降血糖功效与其提高细胞葡萄糖摄取量,抑制α-葡萄糖苷酶活性有关。
英文摘要:
The effects ofCyclocarya paliurus(Batal.) Iljinskaja Extracts on glucose consumption of insulin resistance HepG2 cell and activity of alpha-glucosidase is studied. HepG2 cell with high concentration insulin(10 μg/mL) is induced to establish insulin resistance cell model and divided model cell into Cyclocarya paliuruspolysaccharide(CPP),Cyclocarya paliurusflavone(CPF) and dimethylbiguanide groups. After 24 h administration,glucose assay is used fit to measure the gross glucose consumption of cells(ΔGC), glucose consumption of unite cell(ΔGC/OD) is detected by MTT assay. The inhibitory activity on alpha-glucosidase of CPP and CPF is evaluated,compared with acarbose,in vitro. Compared with model group,ΔGC and ?驻GC/OD of IR-HepG2 cell is increased by CPF and CPP significantly. CPP and CPF have inhibitory effect on the activity of alpha-glucosidase. Those results indicat that CPF and CPP have effects on increasing the glucose consumption of cell and decreasing the activity of alpha-glucosidase,thus alleviating hyperglycemia.
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