牛血清蛋白磁性壳聚糖微球谷氨酸改性吸附

Study of Adsorption Capacity of L-Glutamic Acid-Modified Magnetic Chitosan Microspheres on BSA

DOI：10.3969/j.issn.1673-1689.2017.12.015

中文关键词: 磁性壳聚糖微球 谷氨酸 牛血清蛋白 吸附效果

英文关键词: magnetic chitosan microspheres,L-glutamic acid,bovine serum albumin,adsorption effect

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中文摘要:

研究谷氨酸改性磁性壳聚糖微球对于牛血清白蛋白的吸附。采用反相悬浮法制备谷氨酸改性磁性壳聚糖微球（GA-CMNs），利用红外光谱仪检测壳聚糖连接谷氨酸效果、扫描电子显微镜（SEM）观察微球形貌、激光粒度仪检测微球粒径分布；考察不同pH值、NaCl质量分数、BSA质量浓度、吸附时间对谷氨酸改性磁性壳聚糖微球BSA吸附效果的影响，并对吸附行为进行吸附动力学和吸附热力学分析。结果表明：最佳吸附溶液pH值为5，BSA质量浓度、吸附时间与BSA吸附量呈正相关，而NaCl质量分数则相反；谷氨酸改性磁性壳聚糖微球对BSA吸附平衡过程符合动力学准二级方程模型和Langmuir吸附等温线模型，在BSA初始质量浓度为1 mg/mL时有最大平衡吸附值，为83.5 mg/g；经过5次3% NaCl溶液洗脱-吸附操作循环后，洗脱率仍高于90%。谷氨酸改性磁性壳聚糖微球对牛血清蛋白的吸附性能良好。

英文摘要:

This experiment explored the adsorption capacity of L-glutamic acid-modified magnetic chitosan microspheres（GA- CMNs） for BSA. GA-CMNs was prepared by reverse phase suspension method，the effect of chitosan glutamic acid connection was detected by infrared spectrometer. Microsphere morphology was detected by SEM，and the particle size distribution of microspheres was detected by laser particle size analyzer. The effects of different pH value，NaCl concentration，BSA concentrations，adsorption time were studied on BSA with GA-CMNs，and the adsorption behavior was analyzed by adsorption kinetics and adsorption thermodynamics. The results showed that the optimum adsorption pH was 5，it was a positive correlation between adsorption time and concentration of BSA to adsorbing capacity of BSA，while the ionic strength was the opposite. The maximum amount of equilibrium adsorption was 83.5 mg/g when initial concentration of BSA was 1 mg/mL. This adsorption behavior was in accordance with the second dynamic model and Langmuir isothermal absorption models，3% NaCl was used as eluent，and elution rate was still higher than 90% when adsorption- elution cycles 5 times. GA-CMNs has good adsorption effect on BSA.

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