连接肽的设计及在融合蛋白中的应用

Design of Linker Peptides and Its Application in Fusion Protein

DOI：10.3969/j.issn.1673-1689.2015.11.001

中文关键词: 糖苷水解酶 融合蛋白 连接肽

英文关键词: glycoside hydrolase,fusion protein,linker peptide

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中文摘要:

利用基因工程技术改造酶分子已成为现代分子酶工程的一个研究热点，其中基于融合蛋白设计的融合酶技术已逐渐应用于多功能酶的构建中，表现出了重要的理论与应用研究价值。作为重组融合蛋白不可缺少的部分，连接肽已在融合蛋白的稳定性、生物活性等方面表现出重要的作用。根据近年来的最新研究成果，作者总结了自然界中天然连接肽的性质，并对糖苷水解酶融合酶中连接肽的分类及其对融合酶的作用作了归纳和总结，并对连接肽设计的关键问题进行了探讨和展望。

英文摘要:

At present，the genetic engineering technology has become a hot research topic to modify enzyme molecule. Fusion enzyme technique based on the fusion protein design has been used in the construction of multi-functional enzymes and has shown the important potential application and theory values. As an indispensable component of recombinant fusion proteins，linker peptides have been shown to be important in the stability and bioactivity of fusion proteins. According to the latest research progress in recent years，the general properties of linkers derived from naturally-occurring multi-domain proteins，the classification and the advantageous of the linkers are summarized in this review. Finally，the key problems in the design of the linker and perspectives of this field were also discussed.

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