黑曲霉-葡萄糖苷酶的分离纯化及酶学性质

Purification and Characterization of β-Glucosidase from Aspergillus niger

DOI：10.3969/j.issn.1673-1689.2015.11.013

中文关键词: β-葡萄糖苷酶 对硝基苯-β-D吡喃葡萄糖苷 分离纯化 酶学性质

英文关键词: β-glucosidase,pnitrophenyl β-D-glucopyranoside,purification,enzymatic properties

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中文摘要:

“绿色资源”纤维素广泛应用于能源、医药、食品等领域，β-葡萄糖苷酶（BGL）作为纤维素降解酶系的限速酶是当前研究热点之一。作者对黑曲霉利用豆渣发酵所产的胞外β-葡萄糖苷酶进行分离纯化，并对该酶进行了酶学性质表征。实验结果表明，BGL最适反应温度为55 ℃，最适反应pH为2.0~2.5，pH稳定范围为2.0~7.5。在最适反应条件下，Km值为8.4×10-4 mol/L，kcat为16 s-1，催化效率kcat/Km为1.92×104 L/（mmol?s）。相比较其他报道的BGL酶，该酶具有更好的耐酸性能，可为BGL的理论研究与酶制剂的生产应用提供一定基础。

英文摘要:

Cellulose is one of the most popular-used biomass resource and widely applied in the bioethanol production，medicine，and food industry. The β-glucosidase（BGL） is of interest as the key enzyme in conversion of cellulose to glucose. In this study，glucosidase from Aspergillus niger in bean dregs broth was purified and characterized. The optimum temperature was 55 ℃，and the optimum pH was 2.0~2.5. BGL was stable at pH 2.0~7.5，The Km value was 8.4×10-4 mmol/L，the kcat value was 16 s-1 and the catalytic efficiency（kcat/Km） was 1.92×104 L/（mmol?s） in the optimum condition. This study could promote the theoretical research and the production and application of BGL.

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