野蔷薇根多糖超声微波酶解协同提取及抗氧化活性

Ultrasonic-Microwave-Enzyme Synergistic Extraction of Polysaccharides from Rosa multiflora Thunb and Antioxidant Activity

DOI：10.3969/j.issn.1673-1689.2017.11.009

中文关键词: 野蔷薇根 多糖 超声微波酶解提取 响应曲面法 抗氧化性

英文关键词: Rosa multiflora Thunb,polysaccharides,ultrasonic-microwave-enzymatic,antioxidant activity

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中文摘要:

采用超声-微波酶解协同提取法提取野蔷薇根多糖，以单因素试验为基础，结合响应面试验优化野蔷薇根多糖的提取工艺，并采用化学方法对野蔷薇根多糖进行体外抗氧化活性测定，并与VC进行了比较。结果表明，野蔷薇根多糖的最佳提取工艺条件为：纤维素酶添加质量分数1.15 %（酶活为40 U/mg）、pH值5.72、提取温度81.19 ℃、液料比12.56∶1（mL/g）。在此条件下，野蔷薇根多糖的实际产率为10.48 mg/g，与模型预测结果（10.63 mg/g）基本相接近。抗氧化试验测定结果表明，野蔷薇根多糖具有一定的抗氧化活性。野蔷薇根多糖的抗氧化性略低于VC，并在一定浓度范围内抗氧化活性与多糖含量呈正相关系。

英文摘要:

Extract poiysaccharides from Rosa multiflora Thunb（RMTP） by ultrasonic-microwave-enzyme synergistic extraction method. Based on single factor experiments，response surface methology was combined to optimize the process，and test its in vitro antioxidant activity in a chemical way，and compared with the antioxidant activity of VC. Results showed that optimal process conditions to extract Rosa multiflora Thunb polysaccharides were ：the amount of enzyme added was 1.15%（enzyme activity was 40 U/mg），pH value was 5.72，extraction temperature was 81.19 ℃ and the ratio to liquid was 12.56∶1（mL/g），at this conditions，actual yield of Rosa multiflora Thunb polysaccharides was 10.48 mg/g，it conforms to prediction results of the model（10.63 mg/g）. The results of antioxidant activity showed that the polysaccharides from Rosa multiflora Thunb had strong antioxidant capacity. The polysaccharides from Rosa multiflora Thunb had weaker antioxidant activity than VC.The antioxidant activity and polysaccharide content in certain concentration ranges were related.

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