杏鲍菇提取物对小鼠巨噬细胞免疫活性的研究

Effect of Pleurotus eryngii Different Extracts on Immunity Activity of Mice Macrophages

DOI：10.3969/j.issn.1673-1689.2017.11.013

中文关键词: 杏鲍菇 不同极性提取物 小鼠巨噬细胞 免疫调节作用

英文关键词: Keyword： Pleurotus eryngii,different polar extracts,mice macrophage cell,immunoregulatory activity

基金项目:

摘要点击次数: 223

全文下载次数: 170

中文摘要:

本文中以杏鲍菇子实体为原料，将杏鲍菇不同极性的提取物（PEs）对小鼠巨噬细胞Ana-1进行了免疫活性研究。将PEs作用于小鼠巨噬细胞Ana-1，通过MTT法测定其对Ana-1细胞增殖活性的影响，Elisa法测定Ana-1细胞肿瘤坏死因子（TNF-α）和白细胞介素6（IL-6）在不同提取物浓度和不同作用时间的分泌量变化情况，中性红法检测对细胞吞噬活性的影响，Elisa法测定Ana-1细胞表面膜蛋白Toll样受体（TLR2、TLR4）的表达量的变化情况。结果显示：杏鲍菇4种提取物（PEs）对小鼠巨噬细胞Ana-1有不同程度上的免疫刺激作用，甲醇提取物（PEM）表现出了最强的促进细胞增殖和促进细胞因子分泌的活性，在PEM质量浓度为12.5 μg/mL的条件下，能够促进Ana-1细胞增殖作用最强，细胞因子TNF-α和IL-6均出现分泌高峰；但PEM对细胞因子分泌的作用时间不同，PEM作用于Ana-1细胞后，在12 h时出现TNF-α分泌高峰，而IL-6在24 h时出现分泌高峰。此外，PEM可以激活Ana-1，提高细胞吞噬活性，促进细胞表面膜蛋白受体TLR2、TLR4的表达。本研究结果表明了PEM能够通过促进巨噬细胞增殖、诱导细胞因子分泌和提高吞噬活性的方式实现免疫增强功能。

英文摘要:

The present study investigated the immunomodulatory potential of Pleurotus eryngii different polar extracts（PEs） in mice macrophage cell Ana-1，Ana-1 cell proliferative rate were analyzed by MTT assay，cell Ana-1 cytokines secretion（TNF-α and IL-6） were determined by Elisa reaction kit in different extracts concentration and different stimulation time，cell phagocytosis ability was detected by neutral red assay，Toll-like receptors（TLR2 and TLR4） were assayed by Elisa reaction kit. The results showed that，the PEs respectively were shown to be promoter on growth of Ana-1 cell proilferation，in varying degrees. Methanol extracts（PEM） showed the highest promoting Ana-1 cell proliferative activity and cytokines secretion（TNF-α and IL-6）. In concetration of 12.5 μg/mL，the cell proliferative rate and cytokines secretion were reached peak value. After the stimulation by the 12.5 μg/mL PEM，TNF-α reached secretion peak in 12 h and IL-6 reached secretion peak in 24 h，respcetively. Furthermore，PEM could stimulate Ana-1 cell to promote the phagocytosis ability and increase the expressions of TLRs signaling molecules（TLR2 and TLR4）. In conclusion，PEM has significant immunity-stimulating effects by promoting macrophage cell proliferation，inducing cell cytokine secretion and promoting phagocytosis ability.

查看全文 查看/发表评论 下载PDF阅读器