明日叶中4-羟基德里辛和黄色当归醇的提取纯化及鉴定

Extraction，Prification and Identification of 4-Hdroxyderricin and Xanthoangelol，fromAngelica keiskei

DOI：10.3969/j.issn.1673-1689.2018.11.011

中文关键词: 明日叶 4-羟基德里辛 黄色当归醇 正交试验 纯化 鉴定

英文关键词: Angelica keiskei,4-hydroxyderricin,xanthoangelol,orthogonality test,purification,identification

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中文摘要:

明日叶是一种药食两用的绿色蔬菜，极具开发价值。本文以明日叶为原料，首先通过单因素以及正交试验，优化了明日叶中4-羟基德里辛（4-HD）和黄色当归醇（XAG）的提取工艺参数。其次采用乙酸乙酯萃取、硅胶柱层析以及半制备液相相结合的方法对粗提液中的4-HD和XAG进行分离纯化，并对纯化物的结构及纯度进行分析。结果表明：最佳提取条件为：提取2次、提取溶剂80%乙醇、提取温度55 ℃、提取时间90 min、料液比1∶10。此时，4-HD得率为1.82 mg/g，XAG得率为2.12 mg/g。HPLC-MS及NMR分析结果表明：纯化得到的两种物质正是4-HD和XAG，纯度分别为99.08% 和 98.92%。

英文摘要:

Angelica keiskeiis a kind of high nutritional vegetable used as both food and medicine. In this study，single factor experiment and orthogonality test were developed to optimize the extraction process of 4-hydroxyderricin（4-HD） and xanthoangelol（XAG），fromAngelica keiskei. The optimized extraction conditions were as follows：times of extraction，2 times；extract solvent，80% ethanol；extraction temperature，55 ℃；extraction time，90 min；ratio of solid to liquid，1∶10. Under these optimized conditions，the extraction yield of 4-HD and XAG were 1.82 mg/g and 2.12 mg/g，respectively. Further，isolation and purification of 4-HD and XAG by silica gel column and semi-preparative C18 column was also described，and then the structures and purities were identified by HPLC-MS and NMR. Results showed that 4-HD at 99.08% purity and XAG at 98.92% purity were obtained.

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