新疆特色果蔬中乳酸菌分离鉴定及其抑菌活性

Identification and Antibacterial Activity of Lactic Acid Bacteria Isolated from Fruits and Vegetables Collected from Different Areas In XinJiang

DOI：10.3969/j.issn.1673-1689.2018.11.013

中文关键词: 乳酸菌 16S rRNA 系统发育 抑菌活性

英文关键词: lactic acid bacteria,16S rRNA,phylogenetic analysis,antibacterial activity

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中文摘要:

利用MRS，M17等4种培养基对采自新疆不同地区的8种水果中的乳酸菌进行分离鉴定，根据16S rRNA基因序列确定菌株的系统进化关系，并利用牛津杯法筛选具有抑菌活性的乳酸菌。共分离疑似乳酸菌90株，rep-PCR指纹图谱分型选出15株代表菌株，经16S rRNA基因序列分析，它们分别隶属于乳杆菌属（Lactobacillus）8株、明串珠菌属（Leuconostoc）6株、乳球菌属（Lactococcus）1株，共3个属，8个种。利用牛津杯法筛选出8株对枯草芽孢杆菌、单增李斯特菌、金黄色葡萄球菌具有明显抑制作用的乳酸菌，为乳酸菌作为生物防腐剂应用到食品工业中奠定基础。

英文摘要:

The isolation and identification of lactic acid bacteria from 8 kinds of fruits collected from different regions in XinJinag were carried out by using MRS，M17 and other two different culture medium. The phylogenetic relationship was identified based on analysis of the 16s rRNA gene sequences；and the bacteriocin-producing lactic acid bacteria strains were screened by using the Oxford cup method. A total of 90 lactic acid bacteria strains were isolated from all samples，and 15 representative strains were obtained based on the rep-PCR fingerprint classification. According to the 16s rRNA gene sequence analysis，15 representative strains mainly belong to genus ofLactobacillus（8 strains），Leuconostoc（6 strains），Lactococcus（1 strain） respectively. A total of 8 lactic acid bacteria strains can inhibit the growth of Staphylococcus aureus，Bacillus subtilisandListeria monocytogenes，were screened out by using the Oxford cup method，and they have great potential application for their possible use as a natural biopreservatives in the food industry.

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