酸解氧化魔芋葡甘聚糖对齐口裂腹鱼肠道微生物的影响

Effects of Dietary Acidolysis Products of Oxidized Konjac Glucomannan on Intestinal Microflora ofSchizothorax prenanti

DOI：10.3969/j.issn.1673-1689.2018.11.017

中文关键词: 氧化魔芋葡甘露聚糖酸解产物 齐口裂腹鱼 变性梯度凝胶电泳 实时定量PCR 肠道微生物

英文关键词: acidolysis products of oxidized konjac glucomannan,Schizothorax prenanti,DGGE,real-time quantitative PCR,intestinal microflora

基金项目:

摘要点击次数: 88

全文下载次数: 143

中文摘要:

探讨了氧化魔芋葡甘露聚糖酸解产物对齐口裂腹鱼肠道微生物的影响。选取平均体质量为81±2.64 g健康齐口裂腹鱼360尾，随机分为4组（每组3个重复，每个重复30尾），分别为对照组（饲喂基础饲料）、LOKGM-0.8%组（基础饲料+0.8%LOKGM）、LOKGM-1.6%组（基础饲料+1.6%LOKGM）和LOKGM-3.2%组（基础饲料+3.2%LOKGM），试验期75 d（驯养期15 d，正试期60 d）。采用变性梯度凝胶电泳（DGGE）技术对齐口裂腹鱼后肠肠道内容物细菌16S rDNA V3可变区进行菌群多样性分析，利用实时荧光定量PCR技术对肠道中嗜水气单胞菌及芽孢杆菌的数量进行定量分析。结果表明：与对照组相比，LOKGM-1.6%与LOKGM-3.2%组后肠肠道菌群多样性显著增加（P0.05）；LOKGM-0.8%组齐口裂腹鱼前肠和中肠嗜水气单胞菌的数量显著减少(P

英文摘要:

To study the effects of dietary acidolysis products of oxidized konjac glucomannan（OKGM） on intestinal microflora ofSchizothorax prenanti. A total of three hundred and sixty healthy fishes with average body weight of 81±2.64 g were randomly divided into 4 groups with 3 replicates and 30 fishes per replicate. The 4 groups were control group（basal diet）、LOKGM-0.8% group（basal diet + 0.8% LOKGM）、LOKGM-1.6% group（basal diet + 1.6% LOKGM）、LOKGM- 3.2% group（basal diet + 3.2% LOKGM），respectively. The experiment lasted for 75 d（15 d for adaptation and 60 d for test）. Diversity of posterior gut microflora was determined based on V3 variable regions of bacteria 16S rDNA by using denaturing gradient gel electrophoresis（DGGE） technique. The number ofAeromonas hydrophilaand Bacillus in the intestinal was quantified by real-time quantitative PCR. The results showed as follows：compared with the control group，the diversity of posterior intestinal microflora was significantly increased in the LOKGM-1.6% and LOKGM-3.2% group（P0.05）；the number ofAeromonas hydrophilain anterior and middle intestinal ofSchizothorax prenantiwas significantly decreased in the LOKGM-0.8% group（PAeromonas hydrophilain anterior，middle and posterior intestinal was all significantly decreased in LOKGM-1.6% group（PAeromonas hydrophilain anterior and posterior intestinal was significantly decreased in LOKGM-3.2% group（PSchizothorax prenantiand keep the fish healthy.

查看全文 查看/发表评论 下载PDF阅读器