β-紫罗兰酮温敏脂质体的制备工艺及配方优化

Optimization of Preparation and Formula for Beta-Ionone Liposome

DOI：10.3969/j.issn.1673-1689.2017.10.003

中文关键词: β-紫罗兰酮 脂质体 二棕榈酰磷脂酰胆碱DPPC 氢化大豆卵磷脂HSPC

英文关键词: beta-ionone,liposomes,DPPC（1,2-dipalmitoyl-sn-glycero-3-phosphocholine）,HSPC（hydrogenated soy phosphatidylcholin）

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中文摘要:

以合成磷脂DPPC和氢化大豆卵磷脂HSPC为膜材，采用薄膜超声法和乙醇注入法制备β-紫罗兰酮脂质体，并对其超声条件及配方进行优化。优化得到的较佳工艺参数为：超声功率240 W，超声时间4 min。通过单因素实验优化得到的β-紫罗兰酮脂质体的较佳配方为：DPPC与HSPC摩尔比为8∶2，磷脂质量浓度10 mg/mL，β-紫罗兰酮与Tween80、磷脂的质量比为1∶1∶20。该条件下制备的脂质体包封率为（60.62±3.48） %，平均粒径在200 nm左右，在原子力显微镜的观察下为球状或近似球状的小囊泡。

英文摘要:

To prepare beta-ionone liposomes bythin-film evaporation and ethanol injection using DPPC（1，2-dipalmitoyl-sn-glycero-3-phosphocholine） and HSPC（hydrogenated soy phosphatidy- lcholine） as membrane materials，as well as optimize its preparation formula.The results showed that the optimal preparation condition and formula of beta-ionone liposomes was as follows：the molar ratio of DPPC to HSPCwas 8∶2，the concentration of phosphatidylcholine（PC）was 10 mg/mL，the mass ratio of beta ionone toPCs was 1∶20，the ratio of Tween80 to PCs was 1∶20，the ultrasonic power was 240 W，the time was 4 min.Under these conditions，entrapment efficiency of beta-ionone liposomes was 60.62%±3.48%. The diameters of beta-ionone liposomes were about 200nm，the appearances ofbeta-ionone liposomes were completely or approximately globular vesicles under atomic force microscope（AFM）.

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