百合助眠活性组分的筛选和分析

Screening and Analyzing of the Hypnotic Active Ingredients of Lilium lancifolium Thunb

DOI：10.3969/j.issn.1673-1689.2017.10.015

中文关键词: 百合 助眠 有效成分

英文关键词: Lilium lancifolium Thunb,hypnotize,active ingredients

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中文摘要:

对百合助眠的活性组分进行筛选并对有效成分进行分析。用直接睡眠实验和延长戊巴比妥钠睡眠时间实验对百合助眠作用的有效成分进行研究；用大孔树脂、MCI等方法分离具有助眠作用的有效成分；用核磁共振对有效成分进行结构鉴定。百合的正丁醇提取物能显著增加小鼠睡眠率、睡眠时间，促进小鼠更快进入睡眠。经分离，得到的主要物质结构为（25R）-3β，17α-二羟基-5α-螺甾烷-6-酮-3-O-α-L-鼠李糖基-（1→2）-β-D-葡萄糖苷和（25R）-3β-羟基-5α-螺甾烷-6-酮-3-O-α-L-鼠李糖基-（1→2）-β-D-葡萄糖苷。百合的正丁醇提取物具有显著助眠作用，其中具有助眠作用的主要物质为甾体皂苷。

英文摘要:

To screen and analyze the hypnotic active ingredients of Lilium lancifolium Thunb. Methods：Direct sleep experiment and prolonged pentobarbital sleeping time experiment are used to study the hypnotic active ingredients of Lilium lancifolium Thunb. The hypnotic active ingredients are separated by separation column like macroporous resin column and MCI column. Magnetic resonance imaging（MRI） is used to identify the structure of active ingredients. The n-butanol extract of Lilium lancifolium Thunb can increase the rate and time of sleep，accelerate the sleep of mice. By separating，the main structure of the active ingredients are（25R）-3β，17α-dihydroxy-5α- spirostan-6-one-3-O-α-L-Rhamnopyranosyl-（1→2）-β-D-glucopyranoside and（25R）-3β-hydroxy- 5α-spirostan-6-one-3-O- zα-L-Rhamnopyranosyl-（1→2）-β-D-glucopyranoside. The n-butanol extract of Lilium lancifolium Thunb has significant hypnotic effect and the structure of its main ingredients are saponins.

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