复合酶解法优化黄精多糖提取工艺

Optimization of Multi-Enzymatic Extraction Process of Polysaccharide from Ploygonatum sibiricum

DOI：10.3969/j.issn.1673-1689.2017.09.016

中文关键词: 黄精 多糖 复合酶提取 正交试验

英文关键词: Ploygonatum sibiricum Red,polysaccharide,multi-enzymatic extraction,orthogonal test

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中文摘要:

采用复合酶解法优化黄精多糖提取工艺，苯酚-浓硫酸显色法测定黄精多糖质量浓度，以黄精多糖提取率为指标，对复合酶种类和配比进行筛选后，在单因素试验基础上，考察酶解温度、pH、料液比、加酶量对提取率的影响，并通过正交试验进行优化。结果表明，复合酶提取优于单酶提取和普通水提。酶用量配比为纤维素酶：木瓜蛋白酶=3∶7。酶解最佳条件为：pH值5.0，酶解温度50 ℃，料液比（g/mL）1∶20，加酶量1.5 g/dL，即纤维素酶0.45 g/dL，木瓜蛋白酶1.05 g/dL，酶解2 h后，沸水浸提2 h。在此工艺条件下，黄精多糖提取率可达21.55%，是普通水提法得率的2.75倍，比单酶水解高出12.06％。

英文摘要:

Method of multi-enzyme was used to optimize the extraction process of polysaccharide from Ploygonatum sibiricum，phenol-sulfuric acid colorimetric method was applied to determine the content of polysaccharide，defined the polysaccharide extraction rate as the index to choice the kind and ratio of enzymes，the experimental factors were selected by single factor trials such as enzymatic hydrolysis temperature，pH，the rate of material to solvent，the amount of enzyme concentration，the important ones of which would be chosen to optimize by orthogonal test. Results showed that multi-enzyme extraction was better than extraction by single-enzyme or water. The rate of cellulose to papain was 3 to 7. The optimum enzymolysis conditions were as following：pH value of 5.0，temperature of 50 ℃，the rate of material to solvent was 1 to 20，the amount of enzyme concentration was 1.5%，which was 0.45% of cellulose and 1.05% of papain，2 hour-hot water extraction after 2 hour-enzymatic hydrolysis. In this condition，the maximum polysaccharide rate was 21.55%，which was 2.75 times as high as water，and had 12.6% higher than that of single factor trials.

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