色氨酸合成酶基因工程菌合成S-甲基-L-半胱氨酸

Enzymatic Synthesis of S-methyl-L-cysteine Catalyzed by Tryptophan Synthase Genetically Engineered Microorganism

DOI：10.3969/j.issn.1673-1689.2018.06.013

中文关键词: 色氨酸合成酶 S-甲基-L-半胱氨酸 L-丝氨酸 甲硫醇

英文关键词: tryptophan synthase,S-methyl-L-cysteine,L-serine,methanthiol

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中文摘要:

通过密码子优化设计构建色氨酸合成酶基因工程菌，单因素以及响应面法研究色氨酸合成酶基因工程菌制备S-甲基-L-半胱氨酸。结果表明，温度39 ℃、pH 8.5、L-丝氨酸浓度360 mmol/L，反应平衡时间20 h，S-甲基-L-半胱氨酸的质量浓度为40.49 g/L。

英文摘要:

Tryptophan synthase genetically engineered microorganism was constructed by codon optimization in Escherichia coli. Preparation of S-methyl-L-cysteine from L-serine and methanthiol catalyzed by tryptophan synthase genetically engineered microorganism was investigated by response surface methodology and single-factor experiments. The optimal temperature and pH value were 39 ℃ and 8.5 respectively. The optimal substrate concentration of L-serine was 360 mmol/L. Under the optimal conditions of tryptophan synthase genetically engineered microorganism，the content of S-methyl-L-cysteine was 40.49 g/L after 20 h.

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