超高压对解脂耶氏酵母脂肪酶酶活的影响

Effect of High Hydrostatic Pressure Treatment on the Enzyme Activity of Lipase Lip2 from Yarrowia lipolytica

DOI：10.3969/j.issn.1673-1689.2017.05.012

中文关键词: 超高压 脂肪酶 活性

英文关键词: high hydrostatic pressure,lipase,enzymatic activity

基金项目:

摘要点击次数: 257

全文下载次数: 656

中文摘要:

研究了超高压体系中，压力、温度、pH和保压时间对解脂耶氏酵母脂肪酶活性的影响。结果表明：在超高压作用下，解脂耶氏酵母脂肪酶的活性有所提高；经正交试验优化得到在压力450 MPa、温度45 ℃、pH 7.5、保压时间10 min条件下，脂肪酶活性最高，为同等条件下常压处理的脂肪酶活性的220%。经高压处理后，脂肪酶的最适pH 较常压条件下向碱性方向移动0.5。

英文摘要:

The study investigated the effects of pressure，temperature，dwell time and pH on the enzyme activity of lipase Lip2 from Yarrowia lipolytica（YLLIP2） in high hydrostatic pressure（HHP） treatment. Results showed that HHP improved the activity of YLLIP2 and pressure，temperature，dwell time and pH had significant effects on the enzyme activity. Optimal parameters were found by orthogonal test to be pressure 450 MPa，temperature 45 ℃，dwell time 10 min and pH 7.5. The optimal pH was increased 0.5 in HHP.

查看全文 查看/发表评论 下载PDF阅读器