双酶法催化玉米淀粉制备γ-CD

γ-CD Catalyzed from Maize Starch by Dual Enzymatic Reaction

DOI：10.3969/j.issn.1673-1689.2017.04.004

中文关键词: 4α-糖基转移酶 环糊精葡萄糖基转移酶 γ-环糊精 玉米淀粉

英文关键词: 4α-glycosyltransferase,cyclodextrin glycosyltransferase,γ-cyclodextrin,maize starch

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中文摘要:

以玉米淀粉为底物，研究了来自于栖热水生菌的4α-糖基转移酶（4αGTase）和来自于嗜碱芽孢杆菌的环糊精葡萄糖基转移酶（CGTase）作用于淀粉制备γ-CD的影响因素。结果表明：两种酶的添加方式为先加入4αGTase、再加入CGTase；γ-CD的最佳制备条件为底物质量分数5%，4αGTase加酶量4 U/g淀粉，CGTase加酶量8U/g淀粉，反应时间30 h。在此条件下γ-CD的得率最高为12.83%，比对照组提高了76.7%。

英文摘要:

Theconjunction of 4α-glycosyltransferase（4αGTase） with cyclodextrin glycosyltransferase（CGTase） were screened for their ability ofproducing γ-cyclodextrin（CD） usingmaize starch as substrate. The studies on the influence factors showed that 4αGTase should be added first，followed byCGTase. The optimal conditions for γ-CD production was as following：4U of 4αGTase and 8U ofCGTase per gram of substratewere used to convert maize starch（5%，w/v） into γ-CD with the reaction time of 30 h. the subsequent yield of γ-CD was 12.83%，which was 76.7% higher than the control. This study has potential value for the industrial production of γ-CD.

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