余甘子清除口臭作用的电子鼻评价方法研究

Evaluation Assessment of Oral Odor Inhibition of Phyllanthus emblica L. Using Electronic Nose

DOI:10.3969/j.issn.1673-1689.2017.04.017

中文关键词: 电子鼻 口臭 余甘子

英文关键词: electronic nose,oral odor,Phyllanthus emblica L.

基金项目:

作者

单位

刘伯科

广西大学 轻工与食品工程学院广西 南宁 530004

秦溪

广西大学 轻工与食品工程学院广西 南宁 530004

张晓栋

广西大学 轻工与食品工程学院广西 南宁 530004

林仲仪

广西大学 轻工与食品工程学院广西 南宁 530004

刘小玲

广西大学 轻工与食品工程学院广西 南宁 530004

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中文摘要:

建立基于电子鼻技术口腔恶臭味的检测评价方法。采用电子鼻对口腔唾液不同孵育时间的恶臭味进行了测定,对测定数据进行SPSS、PCA、LDA、Loadings分析及雷达图特征性分析,获得电子鼻应用于口腔恶臭味评价的最佳检测时间、特征参数和表征传感器。进一步比较了电子鼻评价法、感官评价法和蛋白质沉淀-分光光度法三者的相关性,并采用3种方法分析了余甘子提取物对口腔唾液恶臭味气体的影响。各传感器在最大值点附近5 s区分度较好,因此作为特征提取参数。唾液孵育12 h时,传感器R6(W1S)、R7(W1W)和R9(W2W)与其他传感器有显著性差异(P<0.05),其响应值与感官评价值的相关系数均大于0.98。在口臭的检测和评估中,电子鼻评价法、蛋白质沉淀法和感官评价法结果具有一致性。口腔恶臭味的表征传感器为R6(检测甲基类化合物)、R7(检测无机硫化物)和R9(检测有机硫化物),电子鼻评价法可用于抑制口臭活性物质追踪,余甘子提取物具有抑制口臭作用。

英文摘要:

An electronic nose(eNose) analysis was established to determine oral odor in this research. Saliva stench of different incubation times was detected by eNose. The optimal detection time,characteristic parameters and main sensors were obtained via the experimental data from SPSS,PCA,LDA,Loadings analysis and radar map characteristic analysis. The correlation among eNose measurement,sensory evaluation and protein precipitation-spectrophotometry method were compared and analyzed. The impact of Phyllanthus emblica L. extracts on saliva odor was determined by the above three methods. Each sensor was sufficiently distinguished in vicinity 5 s of maximum,which was thus used as the characteristic extraction parameters. There was significant difference (P<0.05) between R6 (W1S),R7 (W1W),R9 (W2W) and the other sensors with 12 h incubation. The correlation coefficients between their response values and sensory evaluation values were larger than 0.98. Consistent results of the oral malodour examination and evaluation were achieved for all three methods. The characteristic sensors of oral odor were R6 detecting methyl compounds,R7 detecting inorganic sulfide and R9 detecting organic sulfide. The eNose can be used to evaluate the inhibition of oral odor. And Phyllanthus emblica L. extracts showed inhibition on oral odor.

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