高效液相色谱法测定酶法制备L-色氨酸

Study on High Performance Liquid Chromatography Method for Determination of Enzymatic Preparation of L-Tryptophan

DOI：10.3969/j.issn.1673-1689.2017.03.016

中文关键词: 高效液相色谱 L-色氨酸 酶法制备

英文关键词: high performance liquid,L-tryptophan,enzymatic preparation

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中文摘要:

研究了高效液相色谱法测定酶法制备L-色氨酸的最佳参数。结果表明，流动相分配比V（磷酸二氢钾溶液）∶V（甲醇）=30∶70，流量1 mL/min，C18柱分离，柱温38 ℃，检测波长265 nm时为高效液相色谱法检测L-色氨酸的最佳参数。回收率在92.00%~110.00%，外加丝氨酸没有干扰发酵液中L-色氨酸含量测定。此方法用于样品中的L-色氨酸的测定，分离效果良好。

英文摘要:

High performance liquid chromatography method for determination of enzymatic Preparation of L-tryptophan（L-Trp） were studied. The results showed that the optimal parameter were 0.05 mmol/L potassium dihydrogan phosphate - methanol （30∶70） as the mobile phase，the detection wavelength by 265 nm，the flow rate of 1.3 mL/min，separated on a C18 column and 38 ℃ column temperature. The recoveries ranged from 92.00%~110.00%，and the Serine added had no interference to determination of L-tryptophan. The L-Trp in fermentation liquid was separated effectively in these conditions.

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