小分子化合物非竞争免疫检测方法研究概述

A Review of Non-Competitive Immunoassays for Small Molecule Compounds

DOI：10.3969/j.issn.1673-1689.2017.02.001

中文关键词: 小分子 非竞争免疫 半抗原

英文关键词: small molecule compounds,non-competitive immunoassay,hapten

基金项目:

摘要点击次数: 173

全文下载次数: 238

中文摘要:

农兽药、非法添加剂及其他有毒有害化学污染物在食品和环境中的残留引起人们越来越多的关注。这些残留物多为小分子化合物，对其进行免疫测定因受限于分子量和抗原表位数量，多用竞争法，通常不能像检测大分子抗原一样采用夹心式的非竞争法，这就制约了其检测的灵敏度、稳定性及工作范围等。但非竞争免疫分析法也并非完全不适用于小分子化合物，这需要方法学上的改进或创新。作者从检测方法原理、体系构成及应用现状等方面综述了近几十年来有关小分子化合物非竞争免疫分析方法的研究成果，以期为相关研究者提供借鉴和参考。

英文摘要:

Pesticides，veterinary drugs，illegal additives and poisonous and harmful chemical pollutants in foods and environment have aroused more and more concerns. These residues are mostly assayed by competitive immunoassay modes due to their small molecular weight and single epitope. Since the sensitivity，stability，and liner range of competitive immunoassays are not as good as those in non-competitive sandwich immunoassays，methodological improvements could made non-competitive immunoassays suitable. Herein this review summarized their principles，elements and applications of non-competitive immunoassays for the detection of small molecules during last decades.

查看全文 查看/发表评论 下载PDF阅读器