海洋细菌Planococcus rifietoensis发酵产色素培养基的优化
Optimization of Fermentation Medium for the Production of Pigments by Marine Bacteria Planococcus rifietoensis
DOI:10.3969/j.issn.1673-1689.2017.02.010
中文关键词: 海洋细菌 响应面优化 色素
英文关键词: marine bacteria,response surface optimization,pigment
基金项目:
作者
单位
孔菲
江南大学 生物工程学院,江苏 无锡 214122
江南大学 工业生物技术教育部重点实验室,江苏 无锡214122
国家海洋局第三海洋研究所 海洋生物遗传资源重点实验室,福建 厦门 361005
蔡宇杰
许晨
许建中
夏金梅
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中文摘要:
在单因素的基础上,以Plackett-Burman(PB)设计结合响应面(RSM)分析法,对产色素海洋细菌Planococcus rifietoensis发酵培养基的8个营养成分配比进行优化。结果表明:培养基最佳碳氮源分别是葡萄糖和蛋白胨,葡萄糖、MgSO4·7H2O和酵母粉添加量显著影响色素的产量。Box-Benhnken设计分析确定最优培养基配方为:葡萄糖9.78 g/L,蛋白胨 8.00 g/L,酵母粉2.05 g/L,MgSO4·7H2O 8.17 g/L,Na2HPO4 0.1 g/L,FeSO4·7H2O 0.005 g/L,NaCl 10 g/L,CaCl2 0.1 g/L,培养54 h,在此培养条件下,色素的OD值可达0.984,比优化前提高了245%。
英文摘要:
Based on monofactorial experiments,Plackett-Burman(PB) design and response surface methodology(RSM) were applied to optimize 8 medium components for the production of pigments by marine bacteria strain Planococcus rifietoensis. Results showed that optimal carbon and nitrogen sources of the medium were glucose and peptone,respectively,and glucose,yeast extract and MgSO4·7H2O had major effects on the production of pigments. The optimal culture medium was comprised of glucose 9.78 g/L,peptone 8.00 g/L,yeast extract 2.05 g/L,MgSO4·7H2O 8.17 g/L,Na2HPO4 0.1 g/L,FeSO4·7H2O 0.005 g/L,NaCl 10 g/L and CaCl2 0.1 g/L. The incubation time was 54 hours. Under these conditions,the optical density(OD) of pigments reached to 0.984,which was 245% higher than that before optimization.
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