载纳米银丝蛋白/海藻酸钠海绵的制备及表征

Preparation and Characterization of Nano-Silver Loaded Fibroin/Alginate Sponge

DOI：10.3969/j.issn.1673-1689.2017.02.013

中文关键词: 生物敷料 抗菌活性 丝蛋白 海藻酸 纳米银

英文关键词: biologic dressing,anti-bacteria potential,silk fibroin,alginate,nano-silver

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中文摘要:

以丝蛋白（SF）和海藻酸钠（SA）为基质构建新型负载纳米银生物敷料。以SF溶液为稳定剂，采用紫外光照射法合成了纳米银，加入SA后，经氯化钙/乙醇/水（摩尔比，1∶71∶77）处理3 h，将上述材料润洗冻干后得到负载纳米银的SF/SA复合海绵（AgNPs-SF/SA）。透射电子显微镜（TEM）观察显示，合成的纳米银平均粒径为（5.03±1.42） nm，在丝蛋白溶液中分布均匀。扫描电子显微镜（SEM）图像显示，与纯丝蛋白海绵相比，AgNPs-SF/SA显示连通的多孔结构。SA的加入增强了海绵的吸水性和保水性；在SF和SA的协同作用下，AgNPs-SF/SA的水蒸气透过率有所提高。以K-B扩散法和菌液培养法测定了AgNPs-SF/SA的抗菌性，结果显示，AgNPs-SF/SA对金黄色葡萄球菌和铜绿色假单胞菌的抗菌性优于阳性对照AQUACEL?誖Ag；24 h和48 h的菌液培养显示，AgNPs-SF/SA具有更持久的抗菌活性。综上所述，AgNPs-SF/SA的物理性能和抗菌性能优良，有望作为一种新型的抗菌生物敷料用于预防和治疗创面感染。

英文摘要:

This study aimed to fabricate a novel Ag nano-particles（AgNPs） loaded biologic dressing based on silk fibroin（SF） and sodium alginate（SA）. AgNPs were first synthesized in the stabilizer，SF solution，under UV irradiation，and after the addition of SA，were then treated by CaCl2/ethanol /water（molar ratio，1∶71∶77） for 3 h. After rinsing and freeze-drying，AgNPs loaded SF/SA composite sponge（AgNPs-SF/SA） was obtained. Transmission electron microscopy observation（TEM） showed that AgNPs were evenly distributed in the SF solution with a mean diameter of 5.03 nm. Scanning electron microscopy（SEM） images showed that compared with pure SF sponge，AgNPs-SF/SA had a linked porous structure. Addition of SA improved the water absorption and retention ability of the sponge and the synergistic effect of SF and SA increased the water vapor transmission rate. K-B disk diffusion and bacterial solution culture methods indicated that AgNPs-SF/SA possessed a higher and more continuous anti-bacterial potential than the positive control，AQUACEL?誖 Ag，against P. aeruginosa and S. aureus. In conclusion，the excellent physical and anti-bacterial properties demonstrated that AgNPs-SF/SA could be a novel anti-bacterial biologic dressing for prevention and treatment of wound infection.

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