泰拉霉素完全抗原的合成和鉴定

Synthesis and Identification of Complete Antigen of Tulathromycin

DOI：10.3969/j.issn.1673-1689.2017.01.011

中文关键词: 泰拉霉素 完全抗原 ELISA 鉴定

英文关键词: Tulathromycin,complete antigens,ELISA,identification

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中文摘要:

利用丁二酸酐法对泰拉霉素的羟基衍生化，引入游离羧基，合成泰拉霉素半抗原。采用活泼酯法将泰拉霉素半抗原分别与牛血清白蛋白（BSA）和鸡卵清白蛋白（OVA）偶联，合成人工完全抗原泰拉霉素-BSA和泰拉霉素-OVA。利用LC-MS鉴定衍生化后半抗原的合成，利用荧光光谱、考马斯亮法鉴定完全抗原的合成。用泰拉霉素-BSA免疫小鼠，采用间接ELISA法测定小鼠抗血清效价。泰拉霉素半抗原和BSA的偶联比为17∶1。三免后小鼠抗血清效价可达1∶8 000。

英文摘要:

The complete antigen of Tulathromycin was synthesized in this study. The Tulathromycin haptens were obtained by deriving the mycotoxins with succinic anhydride，introducing the free carboxyl group. The EDC method was applied to conjugate the Tulathromycin haptens to bovine serum albumin （BSA） as an artificial immunogen. The coating antigen Tulathromycin-OVA was achieved in the same way. The synthetic hapten was identified by LC-MS. Tulathromycin-BSA was characterized by fluorescence spectrometry and Bradford method. The antiserum titer was detected by indirect ELISA for BALB/c mice immunized with Tulathromycin-BSA. The conjugated-ratio of BSA was determined as 17∶1. The antiserum titer of the BALB/C mice reached to 1∶8 000 after the third Booster immunization. The complete antigens were successfully synthesized，which laid a foundation for the development of the Tulathromycin ELISA kit.

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