药食用真菌提取物抗氧化活性研究

Antioxidant Activity of Mycelial Extracts From Medicinal Mushrooms

DOI：10.3969/j.issn.1673-1689.2017.01.014

中文关键词: 抗氧化活性 DPPH自由基 超氧自由基 灵芝 虫草被孢

英文关键词: antioxidant activity,DPPH·,·O2-,Ganoderma lucidum,Cordyceps mortierella

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中文摘要:

为了探究猴头菌、蜜环、灵芝、虫草头孢和虫草被孢等5种药食用真菌菌粉中小分子的抗氧化能力，利用正己烷、氯仿、乙酸乙酯和甲醇等4种有机溶剂按极性提取各菌粉，建立了5种利用96微孔板快速检测物质的抗氧化指标的方法，并比较了不同菌粉提取物的抗氧化活性。结果表明，5种菌粉提取物对·O2-均有着良好的清除能力，虫草被孢和猴头菌提取物对DPPH·清除率比较高，在质量浓度达到8 mg/mL时的清除率分别达到94.91%和92.26%。灵芝氯仿提取物在质量浓度为8 mg/mL时，·OH清除率达到90%，接近VC的清除能力。

英文摘要:

The antioxidant activity of mycelial extracts from medicinal mushrooms was investigated. They were from Cephalosporium sinensis，Cordyceps mortierella，Hericium erinaceum，Ganoderma lucidum and Armillaria mellea using N-hexane，chloroform，ethyl acetate and methanol，respectively. The detection of antioxidant activity was established by five methods by microtiter plates with 96 wells. Their antioxidant activities were then compared and assessed. The results showed that all the extracts could effectively scavenge ·O2-. Extracts from C.mortierella and H.erinaceum had strong scavenging activity to DPPH·，showing up to 94.91% and 92.26% at 8 mg/mL，respectively. The scavenging activity to ·OH of extract from G.lucidum by chloroform was up to 90% at 8 mg/mL，which was comparable to that of Vc. It was demonstrated that the antioxidant activity of these extracts from mushrooms was different from each other. More study will carry out to discuss the small active compounds with antioxidant activity in extracts.

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