酶解制备羊胎盘抗氧化肽工艺条件的优化

Preparation of Antioxidant Peptides From Sheep Placenta Byproductsby Enzymatic Hydrolysis

DOI：10.3969/j.issn.1673-1689.2017.01.017

中文关键词: 羊胎盘 酶解 抗氧化肽 响应面法

英文关键词: sheep placenta,enzymatic hydrolysis,antioxidant peptide,response surface methodology

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中文摘要:

对羊胎盘下脚料为原料制备羊胎盘抗氧化肽的工艺进行了研究。以DPPH自由基清除率和水解度为考察指标，分别采用4种蛋白酶酶解制备羊胎盘抗氧化多肽，结果表明木瓜蛋白酶酶解产物的自由基清除率最高且水解度适中，因此木瓜蛋白酶被确定为水解酶。通过单因素试验与二次响应面回归分析对酶解工艺参数进行了优化。最佳酶解条件为：底物质量浓度33 mg/mL、pH 6.4、酶解温度55 ℃、加酶量4 900 U/g、酶解时间120 min，此时羊胎盘蛋白水解度为7.92 %，10 mg/mL酶解产物对DPPH自由基、超氧阴离子自由基与羟基自由基的清除率分别可达92.15 %、93.45 %与46.88%，显示出较好的抗氧化活性。

英文摘要:

This study aimed to prepare antioxidant peptides from sheep placenta byproducts by enzymatic hydrolysis. Four proteases were used and degree of hydrolysis（DH） and DPPH radical scavenging activity were evaluated. Results showed that the hydrolysate by papain possessed the strongest DPPH radical scavenging activity in spite of a moderate DH. Through single factor tests and the response surface regression analysis，the optimal hydrolysis conditions by papain were thus obtained to be substrate concentration 33 mg/mL，pH 6.4，temperature 55 ℃，enzyme dosage 4 900 U/g and hydrolysis time 120 min. With these parameters，the hydrolysis resulted in a DH of 7.92 % and the hydrolysate had strong scavenging activities for radicals DPPH·，O2-·and ·OH with scavenging rates of 92.15 %，93.45 % and 48.88 %，respectively（10 mg/mL）.

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