强化前体（UDP-GalNAc）合成路径提高果糖软骨素的生产

Improving Fructosylated Chondroitin Production by Enhancement of UDP-GalNAc Synthetic Pathway

DOI：10.3969/j.issn.1673-1689.2020.03.010

中文关键词: 大肠杆菌K4 代谢路径 果糖软骨素 代谢工程 发酵工程

英文关键词: Escherichia coliK4,metabolic pathway,fructosylated chondroitin,metabolic engineering,fermentation engineeringorganic acid

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中文摘要:

硫酸软骨素是一种重要的糖胺聚糖，广泛应用于医药、食品、保健品行业。为了提高硫酸软骨素类似物果糖软骨素的生产，通过过量表达磷酸葡糖胺变位酶（GlmM）、葡萄糖胺合酶（GlmS），优化融合蛋白glmM-glmS的表达水平，获得了最优工程菌株E. coliK4-H-glmM-glmS，并确定了最适IPTG浓度(0.4 mmol/L)以及诱导温度(37 ℃)。最后，在5-L罐水平下，借助DO-stat补料模式，果糖软骨素的产量达到了3.99 g/L，较原始菌株提高了108.9%，为工业化生产果糖软骨素奠定了基础。

英文摘要:

Sulfated chondroitin is an important glycosaminoglycans，which is widely used in medical，food and cosmetic fields. To improve the production of fructosylated chondroitin，GlcN synthase（GlmM） and GlcN-6-P synthase（GlmS） were overexpressed，the confusion protein was constructed and a recombinant strainE. coliK4-H-glmM-glmS was obtained. Then，the optimal induction conditions including IPTG concentration and induction temperature were estalished. Finally，with the DO-stat feeding strategy in a 5 L fermenter，the yield was up to 3.99 g/L，which was 108.90% higher than that with wild type strain. The study provides a basis for industrial-scale production of fructosylated chondroitin.

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