碎米荠蛋白制备工艺及抗氧化活性

Preparation of Cardamine Protein and Its Antioxidant Activity

DOI：10.3969/j.issn.1673-1689.2020.01.010

中文关键词: 碎米荠，蛋白质，硒，提取工艺

英文关键词: cardamine,protein,selenium,extraction process

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中文摘要:

以恩施堇叶碎米荠为原料，研究碎米荠硒蛋白的提取工艺，探讨碎米荠蛋白的抗氧化活性，结果表明：提取温度为50 ℃，料液质量体积比为1 g:40 mL，先后用去离子水和0.1 mol/L 的NaOH溶液分别提取8 h。在此工艺条件下蛋白质得率最高，为75.4%，其中水提蛋白质质量分数为26.5%，硒质量分数为1 748μg/g；碱提蛋白质质量分数为52.3%，硒质量分数为1 513μg/g。碎米荠碱提蛋白经Sephadex G-100 凝胶层析柱分离得到两个组分，分别为峰1和峰2，硒质量分数分别为668μg/g和650μg/g，通过研究碎米荠蛋白对羟自由基（·OH）和1-1-二苯基-2-三硝基苯肼自由基（·DPPH）的清除能力，发现碎米荠蛋白均有一定的清除自由基的能力。

英文摘要:

In order to develop and utilize Enshi Cardamine，in this paper，the extraction process of selenoprotein was studied，and the antioxidant activity of selenoprotein was investigated. The results indicated that the optimal conditions for the extraction were as follows: material ratio 1:40 g/mL，the extraction temperature was 50 ℃，the extraction time was 8 h by distilled water and then 8 h by 0.1 mol/L NaOH solution. Under the optimal conditions，the extraction rate of selenopratein was up to 75.4%. The content of water soluble protein was 26.5 g/dg，the selenium content was 1 748μg/g，the content of alkali soluble protein was 52.3 g/dg，and the selenium content was 1 513μg/g. The alkali soluble protein were separated by Sephadex G-100 gel chromatography，and two components were obtained，peak 1 and peak 2. The selenium content of peak 1 was 668μg/g and the selenium content of peak 2 was 650μg/g. By studying the ability of cardamine proteins to scavenge hydroxyl free radicals（·OH）and 1-diphenyl-2-trinitrophenyl hydrazine free radicals（·DPPH)，it was found that the cardamine protein have certain ability to scavenge free radicals.

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